Abstract |
This study investigated the inhibitory effects of costunolide isolated from the leaves of Laurus nobilis L. (Lauraceae) on basophil-mediated allergic reactions and interleukin (IL)-5-mediated B cell growth. The effects of costunolide on β- hexosaminidase (a key parameter of degranulation) release and IL-4 expression in rat basophilic leukemia (RBL-2H3) cells were determined by measuring β- hexosaminidase activity and by semi-quantitative RT-PCR, respectively. The effects of costunolide on Y16 pro-B cell viability and growth were determined by 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay. Costunolide was found significantly to inhibit β- hexosaminidase activity (p < 0.01) and IL-4 transcription in RBL-2H3 cells in a dose-dependent manner. Its 50% inhibitory concentration (IC50 ) was 34 µM, while that of the positive control, ketotifen, was 24 µM (IL-4 mRNA transcription). Moreover, costunolide dose-dependently suppressed pro-B cell growth in IL-5-stimulated Y16 cells. These results provide evidence that costunolide stabilizes mast cells by inhibiting IgE-mediated degranulation and inhibits IL-5-stimulated B cell growth.
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Authors | Tae Joon Kim, Kung-Woo Nam, Bora Kim, Sung-Jin Lee, Ki-Bong Oh, Kyeong Ho Kim, Woongchon Mar, Jongheon Shin |
Journal | Phytotherapy research : PTR
(Phytother Res)
Vol. 25
Issue 9
Pg. 1392-7
(Sep 2011)
ISSN: 1099-1573 [Electronic] England |
PMID | 21674633
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 John Wiley & Sons, Ltd. |
Chemical References |
- Interleukin-5
- Sesquiterpenes
- Interleukin-4
- Immunoglobulin E
- costunolide
- beta-N-Acetylhexosaminidases
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Topics |
- Animals
- Cell Degranulation
(drug effects)
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Immunoglobulin E
- Inhibitory Concentration 50
- Interleukin-4
(metabolism)
- Interleukin-5
- Laurus
(chemistry)
- Mast Cells
(drug effects, physiology)
- Precursor Cells, B-Lymphoid
(drug effects)
- Rats
- Sesquiterpenes
(pharmacology)
- beta-N-Acetylhexosaminidases
(metabolism)
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