Tim1 and Tim3 are not essential for experimental allergic asthma.

Abstract

BACKGROUND:

Initial studies suggested that polymorphisms in Tim1 and Tim3 contribute to the development of airway hyperreactivity (AHR) in an acute mouse model of asthma. This was also mirrored in human genetic studies where polymorphisms in Tim1 and Tim3 have been associated with atopic populations.

OBJECTIVE:

Further studies using anti-Tim1 or -Tim3 antibodies, or Tim fusion proteins, have also suggested that these molecules may function as regulators of type-1 and type-2 immunity. However, their role in the development of AHR and airway inflammation remains unclear. Given the proposed roles for Tim1 and Tim3 in type-1 and type-2 responses, we sought to determine whether these molecules were important in regulating antigen-driven lung allergy and inflammation.

METHOD:

We used Tim1- and Tim3-deficient mice and determined how the development of allergic lung inflammation was affected.

RESULTS:

AHR was induced normally in the absence of both Tim1 and Tim3, although Tim1-deficient mice did show a small but significant decrease in cell infiltration in the lung and blood eosinophilia. Although Tim3 was expressed on CD4(+) T cells in the allergic lung, Tim1 expression was restricted to CD86(+) B cells.

CONCLUSIONS AND CLINICAL RELEVANCE:

Thus, Tim1 and Tim3 are not essential for the induction of the type-2 response in lung allergy. This is contrary to what was proposed in a number of other studies using neutralizing and activating antibodies and questions the clinical relevance of Tim1 and Tim3 for novel allergy therapies.

• Authors: J L Barlow, S H Wong, S J Ballantyne, H E Jolin, A N J McKenzie
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 41 Issue 7 Pg. 1012-21 (Jul 2011) ISSN: 1365-2222 [Electronic] England
• PMID: 21470319 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Blackwell Publishing Ltd.

Chemical References

• B7-2 Antigen
• Havcr1 protein, mouse
• Havcr2 protein, mouse
• Hepatitis A Virus Cellular Receptor 1
• Hepatitis A Virus Cellular Receptor 2
• Membrane Proteins
• Receptors, Virus

Topics

• Animals
• Asthma (immunology, metabolism, physiopathology)
• B-Lymphocytes (immunology, metabolism)
• B7-2 Antigen (metabolism)
• Bronchial Hyperreactivity (immunology, metabolism, physiopathology)
• CD4-Positive T-Lymphocytes (immunology, metabolism)
• Disease Models, Animal
• Hepatitis A Virus Cellular Receptor 1
• Hepatitis A Virus Cellular Receptor 2
• Humans
• Hypersensitivity, Immediate (immunology, metabolism, physiopathology)
• Inflammation (immunology, metabolism, physiopathology)
• Lung
• Membrane Proteins (deficiency, genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Receptors, Virus (deficiency, genetics, metabolism)