Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients.

Abstract

The aim of the present study was to detect antigenic proteins that react specifically with cerebrospinal fluid (CSF)-IgG from oligoclonal IgG bands (OB)-positive multiple sclerosis (MS) patients. To identify such antigenic proteins, we developed a rat brain proteome map using 2-DE and applied it to the immunoscreening of brain proteins that react with CSF-IgG but not with serum-IgG in OB-positive MS patients. After sequential MALDI-TOF mass spectrometry, eight proteins [two neuronal proteins (tubulin β-2 and γ enolase-2), HSP-1, Tpi-1 protein and cellular enzymes (creatine kinase, phosphopyruvate hydratase, triosephosphate isomerase and phosphoglycerate kinase-1)] were identified as candidate antigens in seven MS patients. Reactivity to tubulin was seen in Western blotting in four patients, and CSF-specific anti-tubulin IgG was detected in one patient. In addition, CSF-specific anti-gamma enolase IgG was found in another patient. These findings suggest that intrathecal immune responses may occur against a broad range of proteins in MS.

• Authors: Masashi Nakamura, Atsuo Kuramasu, Ichiro Nakashima, Kazuo Fujihara, Yasuto Itoyama
• Journal: Proteomics. Clinical applications (Proteomics Clin Appl) Vol. 1 Issue 7 Pg. 681-7 (Jul 2007) ISSN: 1862-8346 [Print] Germany
• PMID: 21136723 (Publication Type: Journal Article)
• Copyright: Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.