Abstract | OBJECTIVES: METHODS: RCC cell line 769P was cultured and proteome analysis was subsequently carried out in the culture supernatants. By using sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and tandem mass spectrometry (LC-MS/MS), proteins in the culture supernatants were searched. A MEDLINE search to define the functions of the identified proteins was carried out. RESULTS: Four differentially regulated proteins ( profilin 1, amyloid beta A4 protein [APP], proprotein convertase subtilisin/kexin type 1 inhibitor [ProSAAS], galectin-3- binding protein [LGALS3BP]) were selected. These were not overexpressed in normal kidney tissue or reported in RCC. Their levels were measured through western blotting of normal kidney and RCC tissues. No differences were observed in the expression levels of APP, ProSAAS or LGALS3BP between RCC and normal kidney tissues. Profilin 1 was overexpressed in RCC tissue. On the basis of this observation, an immunohistochemical analysis of profilin 1 in normal kidney and RCC tissues was carried out. In normal tissues, tubules that were sources of RCC stained positive for profilin 1. In RCC tissue, in contrast, the stromal cells in the tumors stained positive. CONCLUSIONS:
|
Authors | Satoru Minamida, Masatsugu Iwamura, Yoshio Kodera, Yusuke Kawashima, Masaomi Ikeda, Hiroshi Okusa, Tetsuo Fujita, Tadakazu Maeda, Shiro Baba |
Journal | International journal of urology : official journal of the Japanese Urological Association
(Int J Urol)
Vol. 18
Issue 1
Pg. 63-71
(Jan 2011)
ISSN: 1442-2042 [Electronic] Australia |
PMID | 21091798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
|
Copyright | © 2010 The Japanese Urological Association. |
Chemical References |
- Biomarkers, Tumor
- PFN1 protein, human
- Profilins
|
Topics |
- Adult
- Aged
- Biomarkers, Tumor
(metabolism)
- Carcinoma, Renal Cell
(genetics, metabolism)
- Cell Line, Tumor
- Female
- Gene Expression Profiling
- Humans
- Kidney Neoplasms
(genetics, metabolism)
- Male
- Middle Aged
- Profilins
(metabolism)
|