Serial changes of lung morphology and biochemical profiles in a rat model of bronchopulmonary dysplasia induced by intra-amniotic lipopolysaccharide and postnatal hyperoxia.

Abstract

AIM:

to investigate serial changes of lung morphology and biochemical profiles in a rat model of bronchopulmonary dysplasia (BPD) induced by intra-amniotic lipopolysaccharide (LPS) administration and postnatal hyperoxia (85%).

METHODS:

we evaluated histological changes of the lungs and compared the levels of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and protein carbonyl in lung tissue on days 1, 7, and 14 after birth in a rat model of BPD.

RESULTS:

the inhibition of alveolarization was sustained in the LPS plus hyperoxia group from day 7 to 14, whereas alveolarization resumed in the hyperoxia group after oxygen exposure was withdrawn at day 7. Administration of LPS alone did not adversely affect lung morphometry. IL-6 levels showed transient overexpression at day 1 in the LPS-treated groups, but decreased at days 7 and 14. VEGF protein levels were elevated in the LPS-treated groups, but not in the hyper-oxia and control groups at days 1, 7, and 14. Exposure to hyperoxia affected protein carbonyl levels in the hyperoxia group at days 7 and 14.

CONCLUSION:

lung injury induced by intra-amniotic inflammation and postnatal hyperoxia may be due to inhibition of alveolarization without recovery even after withdrawal of oxygen.

• Authors: Hyun Ju Lee, Chang Won Choi, Beyong Il Kim, Ee-Kyung Kim, Han-Suk Kim, Jung-Hwan Choi, Myong Jin Lee, Eun Gyeong Yang
• Journal: Journal of perinatal medicine (J Perinat Med) Vol. 38 Issue 6 Pg. 675-81 (11 2010) ISSN: 1619-3997 [Electronic] Germany
• PMID: 20707615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-6
• Lipopolysaccharides
• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Animals
• Animals, Newborn
• Bronchopulmonary Dysplasia (chemically induced, metabolism, pathology)
• Disease Models, Animal
• Female
• Histocytochemistry
• Humans
• Hyperoxia (pathology)
• Infant, Newborn
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• Male
• Pregnancy
• Protein Carbonylation
• Pulmonary Alveoli (drug effects, metabolism, ultrastructure)
• Rats
• Rats, Sprague-Dawley
• Survival Analysis
• Vascular Endothelial Growth Factor A (metabolism)