Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.

Abstract

To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer.

• Authors: Jin-Eun Kim, Won-Yoon Chung, Kyung-Soo Chun, Chang Ki Lee, Hee-Juhn Park, Won-Bae Kim, Kwang-Kyun Park
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 74 Issue 4 Pg. 788-92 ( 2010) ISSN: 1347-6947 [Electronic] England
• PMID: 20378978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• bcl-2-Associated X Protein
• Poly(ADP-ribose) Polymerases
• Caspase 3
• Caspase 9

Topics

• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Cell Survival (drug effects)
• Cells (metabolism, pathology)
• Colonic Neoplasms (metabolism, pathology)
• Flow Cytometry
• HT29 Cells
• Humans
• Mitochondria (metabolism, pathology)
• Poly(ADP-ribose) Polymerases (metabolism)
• bcl-2-Associated X Protein (metabolism)