Abstract | OBJECTIVE: METHODS: C57BL/CN mice were treated with MPTP to prepare a subacute PD model, and their behavioral changes following the treatment were observed. Immunohistochemistry and Western blotting were performed to detect the expression of tyrosine hydroxylase (TH), COX-2 and phosphorylation of P38MAPK in the SN and their changes following treatment with SB203580, a specific inhibitor of P38MAPK. RESULTS: The 7-day model group showed typical symptoms of PD with decrements of TH-positive neurons and TH protein level in the SN of the midbrain by about 65% and 75%, respectively (P<0.01). In the 3-day model group, the COX-2-, caspase-3- and phosphorylated P38MAPK-immunoreactive cells and their protein levels in the SN increased markedly with obvious loss of TH-positive neurons. Administration of SB203580 obviously lessened the above changes (P<0.01). CONCLUSION:
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Authors | Zi-feng Wei, Yong-sheng Wang, Li-ren Ma, Qian Wang, Zuo-feng Zhang, Yu-xin Zhang |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 29
Issue 10
Pg. 2010-3, 2017
(Oct 2009)
ISSN: 1673-4254 [Print] China |
PMID | 19861252
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ptgs2 protein, mouse
- Cyclooxygenase 2
- p38 Mitogen-Activated Protein Kinases
- Casp3 protein, mouse
- Caspase 3
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Topics |
- Animals
- Caspase 3
(genetics, metabolism)
- Cyclooxygenase 2
(genetics, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Parkinson Disease
(metabolism)
- Signal Transduction
- Substantia Nigra
(metabolism)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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