Increased virulence of Zygomycetes organisms following exposure to voriconazole: a study involving fly and murine models of zygomycosis.

Abstract

BACKGROUND:

Breakthrough zygomycosis is increasingly observed among patients at high risk for fungal infection who are receiving voriconazole, reflecting either selective pressure or voriconazole-associated alterations in Zygomycetes virulence. We tested the latter hypothesis, using 2 phylogenetically disparate zygomycosis models.

METHODS:

Three Zygomycetes strains were exposed to voriconazole by serial passages on voriconazole-containing medium. The virulence of voriconazole-exposed Zygomycetes strains was compared with that of voriconazole-nonexposed strains in Drosophila and murine models of zygomycosis by assessment of survival curves, pulmonary fungal burdens, and expression of inflammation-associated genes.

RESULTS:

Among Toll-deficient (Tl(-/-)) and wild-type fruit flies, infection with Zygomycetes isolates that had been exposed to voriconazole yielded significantly lower survival rates than infection with Zygomycetes strains grown in drug-free media. In contrast, exposure of Rhizopus oryzae to itraconazole, amphotericin B, or caspofungin and exposure of Aspergillus fumigatus to voriconazole did not alter the virulence of these isolates in fruit flies. In the murine model, infection with a R. oryzae strain preexposed to voriconazole was associated with decreased survival rates and increased pulmonary fungal burdens, compared with infection with a voriconazole-nonexposed R. oryzae strain. In addition, enhanced angioinvasion, inflammation, and expression of genes involved in stress response and tissue repair were found in mouse lungs infected with voriconazole-exposed R. oryzae.

CONCLUSIONS:

Exposure of Zygomycetes organisms to voriconazole selectively enhanced their virulence. The mechanisms underlying these phenotypic changes should be studied further.

• Authors: Gregory A Lamaris, Ronen Ben-Ami, Russell E Lewis, Georgios Chamilos, George Samonis, Dimitrios P Kontoyiannis
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 199 Issue 9 Pg. 1399-406 (May 01 2009) ISSN: 0022-1899 [Print] United States
• PMID: 19358672 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antifungal Agents
• Pyrimidines
• Triazoles
• Itraconazole
• Amphotericin B
• Voriconazole

Topics

• Amphotericin B (therapeutic use)
• Animals
• Antifungal Agents (pharmacology, therapeutic use)
• Aspergillus fumigatus (drug effects, isolation & purification)
• Disease Models, Animal
• Drosophila (drug effects)
• Fungi (drug effects, isolation & purification)
• Humans
• Itraconazole (therapeutic use)
• Mice
• Mucor (pathogenicity)
• Pyrimidines (pharmacology)
• Rhizopus (pathogenicity)
• Triazoles (pharmacology)
• Virulence (drug effects)
• Voriconazole
• Zygomycosis (drug therapy, pathology, physiopathology)