Abstract | AIMS: MAIN METHODS: In this study, we investigated whether lowering of ADMA by overexpression of dimethylarginine dimethylaminohydrolase (DDAH), a main enzyme that degrades ADMA, could ameliorate glomerular capillary loss and sclerosis in a rat model of CKD. Four weeks after 5/6 subtotal nephrectomy (Nx), animals were given tail vein injections with recombinant adenovirus vector encoding DDAH-I (Adv-DDAH) or control vector expressing bacterial beta-galactosidase (Adv-LZ), or orally administered with 20 mg/kg/day of hydralazine (Hyz) which served as a blood pressure control model. KEY FINDINGS: Plasma levels of ADMA were associated with decreased number of glomerular capillaries as well as severity of glomerular sclerosis in Nx-rats. These glomerular changes progressed in Adv-LZ- or Hyz-treated Nx-rats, while they were ameliorated by the treatment with DDAH overexpression. SIGNIFICANCE: Our present data suggest that ADMA may be involved in glomerular capillary loss and sclerosis, thus contributing to the progression of CKD. Substitution of DDAH protein or enhancement of its activity may become a novel therapeutic strategy for the treatment of CKD.
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Authors | Seiji Ueda, Sho-ichi Yamagishi, Yuriko Matsumoto, Yusuke Kaida, Ayako Fujimi-Hayashida, Kiyomi Koike, Hideharu Tanaka, Kei Fukami, Seiya Okuda |
Journal | Life sciences
(Life Sci)
Vol. 84
Issue 23-24
Pg. 853-6
(Jun 05 2009)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 19351540
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- N,N-dimethylarginine
- Arginine
- Amidohydrolases
- dimethylargininase
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Topics |
- Amidohydrolases
(biosynthesis)
- Animals
- Arginine
(analogs & derivatives, physiology)
- Capillaries
(metabolism, pathology)
- Disease Models, Animal
- Glomerulosclerosis, Focal Segmental
(metabolism, pathology)
- Humans
- Kidney Failure, Chronic
(metabolism, pathology)
- Kidney Glomerulus
(blood supply, metabolism, pathology)
- Rats
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