Abstract |
The neuropathic pain model consisting of the spared nerve injury of the sciatic nerve was used in the mouse to examine whether peripheral neuropathy is capable of generating over-expression of pro-inflammatory and pro-apoptotic genes in the orbito-frontal cortex, together with allodynia and hyperalgesia. RT-PCR analysis showed increased expression of caspase-1, caspase-12 and caspase-8 genes in the orbito-frontal cortex 14 days after spared nerve injury of the sciatic nerve. Conversely, the expression of caspase-3 was decreased by spared nerve injury of the sciatic nerve in the same brain area. A single subcutaneous injection of ozone performed 12 h after the surgical procedure decreased mechanical allodynia and normalized the mRNA caspase-1, caspase-12 and caspase-8 gene levels, but did not the decrease caspase-3 level, 14 days post-spared nerve injury. Ozone also reduced IL-1beta staining in the orbito-frontal cortex in neuropathic mice. This study provides evidence that a single subcutaneous administration of ozone decreased neuropathic pain type behaviour, normalized the expression of pro-inflammatory caspases and reduced IL-1beta staining in the orbito-frontal cortex astrocytes in SNI mice. These preliminary data show that peripheral neuropathy induced over-expression of pro-inflammatory/pro-apoptotic caspases in the orbito-frontal cortex and that ozone, by mechanisms that are as yet unknown, can regulate the expression of the genes that play a pivotal role in the onset and maintenance of allodynia.
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Authors | Carlo Fuccio, Carlo Luongo, Paola Capodanno, Catia Giordano, Maria Antonietta Scafuro, Dario Siniscalco, Biagio Lettieri, Francesco Rossi, Sabatino Maione, Liberato Berrino |
Journal | European journal of pharmacology
(Eur J Pharmacol)
Vol. 603
Issue 1-3
Pg. 42-9
(Jan 28 2009)
ISSN: 1879-0712 [Electronic] Netherlands |
PMID | 19100257
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-1beta
- Ozone
- Caspases
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Topics |
- Animals
- Apoptosis
(genetics)
- Astrocytes
(drug effects, immunology)
- Behavior, Animal
(drug effects)
- Caspases
(genetics)
- Frontal Lobe
(drug effects, enzymology, metabolism)
- Gene Expression Regulation, Enzymologic
(drug effects)
- Hyperalgesia
(drug therapy)
- Inflammation
(enzymology, genetics)
- Injections, Subcutaneous
- Interleukin-1beta
(immunology)
- Male
- Mice
- Ozone
(administration & dosage, pharmacology, therapeutic use)
- Pain
(metabolism, prevention & control)
- Peripheral Nervous System Diseases
(drug therapy, enzymology, genetics)
- Sciatic Nerve
(drug effects, injuries, metabolism)
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