Although
antimicrobial peptides (AMPs) appear to have diverse functional activities in innate immunity, a few reports suggest a potential role of human
beta-defensin (hBD)-1 in
tumor suppression. The aim of the present study was to compare the expression patterns of hBD-1, -2 and -3 in various features of human salivary gland tissues, such as healthy parenchyma,
chronic sialadenitis and intraglandular
pleomorphic adenomas, with their adjacent normal tissues. Twenty human salivary gland specimens (five healthy, five
chronic sialadenitis, five
pleomorphic adenomas and five
adenoma adjacent normal tissues (AANTs)) were investigated for
mRNA expression levels of hBD-1, -2 and -3 by quantitative real-time RT-PCR. Additionally, immunohistochemistry for the hBD-1, -2 and -3
peptides was performed for analysis of localization. Considerably increased, 80-fold higher hBD-1 and increased hBD-3
mRNA expression levels compared to healthy salivary gland tissues were detected in
chronic sialadenitis. In
pleomorphic adenomas hBD-2 expression levels were lower, but hBD-1 expression levels were significant decreased (p=0.03) compared to healthy parenchyma. Interestingly, the AANTs showed a 48-fold higher expression of hBD-1 and increased hBD-3 expression compared to the healthy salivary gland. Immunohistochemistry of the
tumors showed nuclear hBD-1 localization. For the first time, it was shown that hBD-1 gene expression is significantly decreased in
pleomorphic adenomas, while simultaneously the
protein is localized in the nucleus. Increased expression levels in glandular
inflammation have been described previously albeit not in AANTs. These data support the hypothesis that hBD-1 might be a potential
tumor suppressor also in benign salivary gland
tumors in addition to other genetic alterations.