Abstract |
To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA ( mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF], interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6, IL-12alpha, IL-12beta, and gallinacin (Gal)-2 in the ceca of young chicks 1 wk postinoculation with SE. Cecum tissue sections were stained and evaluated for the presence of macrophages, lymphocytes, heterophils, and apoptotic cells following SE infection. With the use of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), SE infection was associated with a significant (P < 0.01) upregulation of cecal CXCLi1 and CXCLi2 mRNA expression. Infection with SE was also associated (P < 0.05) with increased staining for macrophages and decreased apoptosis ( single-stranded DNA [ssDNA]) in cecal tissue sections when these sections were compared with those of uninfected animals. Changes in chemokine expression and cell population dynamics are a direct result of SE infection, as uninfected animals do not show these alterations. Thus, these SE-induced changes reflect the host immune response to SE in young chickens.
|
Authors | Jennifer H Cheeseman, Nyssa A Levy, Pete Kaiser, Hyun S Lillehoj, Susan J Lamont |
Journal | Avian diseases
(Avian Dis)
Vol. 52
Issue 2
Pg. 229-34
(Jun 2008)
ISSN: 0005-2086 [Print] United States |
PMID | 18646451
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- Chemokines, CXC
- RNA, Messenger
|
Topics |
- Animals
- Cecum
(immunology, pathology)
- Chemokines, CXC
(genetics)
- Chickens
- Gene Expression
- Lymphocytes
(pathology)
- Macrophages
(pathology)
- Poultry Diseases
(genetics, immunology, pathology)
- RNA, Messenger
(genetics, metabolism)
- Salmonella Infections, Animal
(genetics, immunology, pathology)
- Salmonella enteritidis
(immunology, pathogenicity)
|