Multiple drug-resistant gene 1 in children with steroid-sensitive nephrotic syndrome.

Abstract

BACKGROUND:

A full dose of corticosteroid is required to induce complete remission (CR) in steroid-sensitive nephrotic syndrome (SSNS), unless it is possible to taper and discontinue along with the course after CR. But the mechanism of this change in steroid sensitivity remains unknown. P-glycoprotein (PGP) can eliminate given corticosteroids from cytoplasm, which results in corticosteroid resistance. Therefore, drug delivery was analyzed using real-time polymerase chain reaction (PCR) of multiple drug-resistant gene 1 (MDR1; encoding PGP) mRNA expression.

METHODS:

Fourteen patients with SSNS (male/female: 14/0; age: 1-23 years; mean 10.4 years) were enrolled in the study. MDR1 mRNA gene expression of peripheral blood nucleated cells (PBNC), before and after CR (19 sets of blood samples), was quantified using real-time PCR.

RESULTS:

The MDR1 mRNA levels before CR were variable in each patient, but there was an apparent decrease in the MDR1 gene expression of PBNC after CR (P < 0.003).

CONCLUSION:

PGP may play a role in the tapering of corticosteroids after CR in SSNS.

• Authors: Satoshi Funaki, Shori Takahashi, Naohiro Wada, Hitohiko Murakami, Kensuke Harada
• Journal: Pediatrics international : official journal of the Japan Pediatric Society (Pediatr Int) Vol. 50 Issue 2 Pg. 159-61 (Apr 2008) ISSN: 1442-200X [Electronic] Australia
• PMID: 18353050 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ABCB1 protein, human
• ATP Binding Cassette Transporter, Subfamily B
• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Adrenal Cortex Hormones
• RNA, Messenger

Topics

• ATP Binding Cassette Transporter, Subfamily B
• ATP Binding Cassette Transporter, Subfamily B, Member 1 (genetics, metabolism)
• Adolescent
• Adrenal Cortex Hormones (therapeutic use)
• Adult
• Child
• Child, Preschool
• Drug Resistance, Multiple (genetics)
• Female
• Humans
• Infant
• Male
• Nephrotic Syndrome (drug therapy, genetics, metabolism)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction