Abstract | OBJECTIVE: PATIENTS, SUBJECTS AND METHODS: The MMP-1 polymorphism was assessed in 102 transitional cell carcinomas of the bladder (50 Ta, 52 T2-4) and in 94 age-, smoking- and gender-matched healthy volunteers. The polymerase chain reaction (PCR) assay was used to determine the MMP-1 genotypes. Genomic DNA used for the assay was extracted from peripheral blood lymphocytes. RESULTS: The frequency of the MMP-1 2G/2G genotype, which results in the highest MMP-1 transcriptional level, was compared to that of the 1G/1G plus 1G/2G genotypes. Of the 102 cases with bladder cancer, 49 (48%) showed the 2G/2G genotype, whereas it was found in 22 of the 94 controls (23%); this difference was statistically significant (P < 0.01; odds ratio 2.79, 95% confidence interval, CI, 1.53-5.60). However, there was no significant association between the 2G/2G genotype and tumour grade and pathological stage. We assessed the interaction between smoking status (former and current smokers, by their median pack years) and 2G/2G genotype; there was a significantly increased risk in heavy smokers (P < 0.001; odds ratio 3.21; 95% CI 1.33-5.60). CONCLUSION: These results suggest that the MMP-1 promoter polymorphism might be linked to susceptibility for bladder cancer.
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Authors | Ali I Tasci, Volkan Tugcu, Emin Ozbek, Bedi Ozbay, Abdulmuttalip Simsek, Vedat Koksal |
Journal | BJU international
(BJU Int)
Vol. 101
Issue 4
Pg. 503-7
(Feb 2008)
ISSN: 1464-410X [Electronic] England |
PMID | 17986285
(Publication Type: Journal Article)
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Chemical References |
- Matrix Metalloproteinase 1
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Topics |
- Carcinoma, Transitional Cell
(epidemiology, genetics)
- Case-Control Studies
- Female
- Genetic Predisposition to Disease
(epidemiology, genetics)
- Genotype
- Humans
- Male
- Matrix Metalloproteinase 1
(genetics)
- Middle Aged
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
(genetics)
- Promoter Regions, Genetic
(genetics)
- Risk Factors
- Smoking
(adverse effects)
- Turkey
(epidemiology)
- Urinary Bladder Neoplasms
(epidemiology, genetics)
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