Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.

Abstract

During late lytic infection of mouse kidney cell cultures polyoma 16S and 19S (late 19S RNA) were isolated by oligo(dT)-cellulose chromatography. Approximately 60-80% of total cytoplasmic polyoma RNA contained tracts of poly(A) which were retained by oligo(dT)-cellulose. Early in lytic infection when viral DNA synthesis and the production of capsid protein are blocked by the addition of 5-fluorodeoxyuridine, approximately 100% of polyoma "early" 19S RNA was quantitatively retained by oligo(dT)-cellulose indicating the presence of poly(A) tracts on most 19S mRNA molecules. In addition, 2 classes polyoma RNA, synthesized after the onset of cellular RNA synthesis under conditions where DNA synthesis is inhibited with 5-fluorodeoxyuridine, were found to contain tracts of poly(A). These species sedimenting at 16S and 19S in aqueous sucrose density gradients were also quantitatively retained by oligo (dT)-cellulose.

• Authors: L J Rosenthal
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 3 Issue 3 Pg. 661-76 (Mar 1976) ISSN: 0305-1048 [Print] England
• PMID: 179059 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• RNA, Neoplasm
• RNA, Viral
• Floxuridine
• Poly A

Topics

• Cell Line
• Cell Transformation, Neoplastic
• DNA Replication (drug effects)
• Floxuridine (pharmacology)
• Nucleic Acid Hybridization
• Poly A (analysis)
• Polyomavirus
• RNA, Messenger (biosynthesis, isolation & purification)
• RNA, Neoplasm (biosynthesis, isolation & purification)
• RNA, Viral (biosynthesis, isolation & purification)