Abstract | BACKGROUND: The invasive interaction between cells and their matrix has important roles in tumor cell invasion. This study investigated modulation of basement membrane (BM) proteins, especially collagen IV (Coll IV), laminin, and fibronectin (FN), in invasion of human pancreatic cancer cells. Furthermore, we examined the roles of beta(1)-integrins and arginine- glycine-aspartic (RGD)-containing oligopeptide in cell-matrix interactions. MATERIALS AND METHODS: RESULTS: BM proteins significantly enhanced the invasive behavior of pancreatic cancer cells. Pretreatment with anti-beta(1)-integrin antibody suppressed invasion into Matrigel, but RGD-containing peptide inhibited invasion, which was enhanced by Coll IV and FN, not laminin. Treatment with both RGD-containing peptide and beta(1)-integrin antibody inhibited ERK1/2 phosphorylation activated by Coll IV and FN. CONCLUSIONS:
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Authors | Hirozumi Sawai, Yuji Okada, Hitoshi Funahashi, Hiroki Takahashi, Yoichi Matsuo, Akira Yasuda, Nobuo Ochi, Hiromitsu Takeyama, Tadao Manabe |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 144
Issue 1
Pg. 117-23
(Jan 2008)
ISSN: 0022-4804 [Print] United States |
PMID | 17688882
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Collagen Type IV
- Extracellular Matrix Proteins
- Fibronectins
- Integrin beta1
- Laminin
- Oligopeptides
- arginyl-glycyl-aspartic acid
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
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Topics |
- Basement Membrane
(metabolism, pathology)
- Cell Line, Tumor
- Collagen Type IV
(genetics, metabolism)
- Extracellular Matrix Proteins
(genetics, metabolism)
- Fibronectins
(genetics, metabolism)
- Gene Expression Regulation, Neoplastic
- Humans
- Integrin beta1
(genetics, metabolism)
- Laminin
(genetics, metabolism)
- MAP Kinase Signaling System
(physiology)
- Mitogen-Activated Protein Kinase 1
(metabolism)
- Mitogen-Activated Protein Kinase 3
(metabolism)
- Neoplasm Invasiveness
(physiopathology)
- Oligopeptides
(genetics, metabolism)
- Pancreatic Neoplasms
(pathology, physiopathology)
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