Abstract |
To make progress toward an efficient gene vector for cancer gene therapy, a novel nonviral vector of polybutylcyanoacrylate nanoparticles (PBCA NPs) was developed. Cetyltrimethyl ammonium bromide ( CTAB) was used to modify the surface of PBCA NPs, and then the plasmid DNA (pDNA) of pAFP-TK was wrapped into PBCA- CTAB NPs. Atomic force microscopy and zeta potential demonstrated that PBCA- CTAB NPs were 80-200 nm in diameter and had +15.6 mV positive surface charges. Assay using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide showed that PBCA- CTAB NPs had less cytotoxicity to 3T3 cells than HepG2 cells. The analysis of PBCA- CTAB- DNA complexes could not only protect DNA from degradation by DNase I, it could also transfer pDNA into targeted cells with high transfection efficiency. Furthermore, when PBCA- CTAB NPs combined with suicide gene pAFP-TK, alpha-fetoprotein-positive cells transfected by it were highly sensitive to ganciclovir treatment, and cell survival declined precipitously. Therefore, this target strategy using a pAFP-TK/GCV suicide gene therapy system in which PBCA- CTAB NPs serve as gene delivery vectors explores a promising area for alpha-fetoprotein-positive hepatocellular carcinoma and associated carcinoma therapy.
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Authors | Yangde Zhang, Yanqiong Zhang, Jiji Chen, Binghua Zhang, Yifeng Pan, Lifeng Ren, Jinfen Zhao, Yulin Luo, Denggao Zhai, Shunwei Wang, Jiwei Wang |
Journal | Nanomedicine : nanotechnology, biology, and medicine
(Nanomedicine)
Vol. 3
Issue 2
Pg. 144-53
(Jun 2007)
ISSN: 1549-9642 [Electronic] United States |
PMID | 17468053
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Coated Materials, Biocompatible
- Drug Carriers
- DNA
- Enbucrilate
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Topics |
- Cell Line, Tumor
- Coated Materials, Biocompatible
(chemistry)
- DNA
(administration & dosage, genetics)
- Drug Carriers
(chemistry)
- Enbucrilate
(chemistry)
- Genetic Therapy
(methods)
- Humans
- Liver Neoplasms
(genetics, therapy)
- Materials Testing
- Nanoparticles
(chemistry, ultrastructure)
- Particle Size
- Transfection
(methods)
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