[Effects of Ginkgo biloba extract 50 on hypoxia induced endothelial dysfunction].

Abstract

OBJECTIVE:

To preliminarily investigate the influence of hypoxia on human umbilical vein endothelial cells (HUVECs), and the effect of Ginkgo biloba extract 50 (GBE50) on it.

METHODS:

Flow cytometry, TUNEL, RT-PCR, Western blot, etc. were applied, to study the effect of hypoxia and GBE50 on endothelial cells.

RESULTS:

After being interfered by hypoxia for 24 h, the levels of reactive oxygen species (ROS) in HUVECs and the apoptotic rate either in the early or in the late stage significantly increased, and accompanied with the increased levels of endothelin-1 mRNA (ET-1) and endothelial oxide synthase (eNOS) protein. However, when HUVECs were pretreated with GBE50 (25 [microg/ml) 4 h before hypoxia, the apoptotic rate in the early or late stage and expression of ET-1 mRNA significantly decreased (P < 0.05), and the heightened ROS level and eNOS expression partially decreased (P > 0.05).

CONCLUSION:

Hypoxia can induce endothelial dysfunction, which could be partially or significantly reversed by GBE50, it shows a certain protective effect on hypoxia induced endothelial dysfunction.

• Authors: Jian-Ying Shen, Ai-Jun Sun, Xiao-Mei Gu
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 27 Issue 2 Pg. 151-4 (Feb 2007) ISSN: 1003-5370 [Print] China
• PMID: 17343004 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Endothelin-1
• Free Radical Scavengers
• RNA, Messenger
• Reactive Oxygen Species

Topics

• Blotting, Western
• Cell Hypoxia
• Cells, Cultured
• Drugs, Chinese Herbal (pharmacology)
• Endothelial Cells (drug effects, metabolism)
• Endothelin-1 (biosynthesis, genetics)
• Endothelium, Vascular (drug effects, metabolism, physiopathology)
• Free Radical Scavengers (pharmacology)
• Ginkgo biloba (chemistry)
• Humans
• Plant Leaves (chemistry)
• RNA, Messenger (biosynthesis, genetics)
• Reactive Oxygen Species (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction