[Cytoplasmic staining pattern characteristic for anti Jo-1 antibody in HEp-2 cells].

Abstract

We wished to confirm the staining pattern of HEp-2 cells by the anti Jo-1 antibody, because we found antibodies in serum with positive anti Jo-1 antibody which showed either a fibrilar cytoplasmic staining or a nuclear speckled staining pattern in indirect immuno fluorescence+ examinations using HEp-2 cells. Sera available from eight patients with PM DM (polymyositis and/or dermatomyositis) showed positive anti Jo-1 antibody in the double immuno-diffusion technique but had various staining patterns of HEp-2 cells in the immunofluorescent examination. We examined these eight sera with the immuno-blotting method utilizing whole cell extract of HeLa cells, and found the 50 kDa band from all sera tested, to which Jo-1 antigen had been reported to move. We eluted the antibody which formed the 50 kDa band from the nitrocellulose membrane and applied it on HEp-2 cells. This maneuver gave us the fine granular cytoplasmic staining of anti Jo-1 antibody on HEp-2 cells. We therefore concluded that the anti Jo-1 antibody should have cytoplasmic staining on HEp-2 cells although observers might miss it due to other types of associated antinuclear antibodies.

• Authors: Y Nagano, S Yoshinoya, A Ohkubo
• Journal: Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 39 Issue 3 Pg. 269-72 (Mar 1991) ISSN: 0047-1860 [Print] Japan
• PMID: 1711130 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Antinuclear
• Jo-1 antibody

Topics

• Antibodies, Antinuclear (analysis)
• Cytoplasm
• Fluorescent Antibody Technique
• Humans
• Immunodiffusion
• Myositis (diagnosis)
• Staining and Labeling
• Tumor Cells, Cultured