Abstract | BACKGROUND: METHODS: We cloned and sequenced the full-length cDNA for GPx-1 (GenBank: AY966403) and GPx-4 (GenBank: AY966404) from the common marmoset (Callithrix jacchus) in order to create a suitable model for studying human diseases related with oxidative stress. RESULTS: The cDNAs encode a 202 amino acid protein for GPx-1 and a 197 amino acid protein for GPx-4. Both proteins include selenocysteine (Sec, in Gpx-1 at position 48; in GPx-4 at position 73) and showed high homology (>90%) with other mammalian GPxs. The relative levels of mRNA expression for GPx-1 and GPx-4 were determined in different marmoset tissues by quantitative real-time reverse transcriptase-polymerase chain reaction using transcription elongation factor-2 as a reference gene. GPx-1 showed increased levels of expression in the liver, heart and kidney while the highest mRNA levels for GPx-4 were detected in the testis, followed by the liver, lung, kidney and spinal cord. CONCLUSIONS: These findings will be of value for studies designed to assess the role of glutathione peroxidases in non-human primate models for a variety of diseases in which increased oxidative stress has been implicated.
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Authors | Srebrena Atanasova, Nicolas von Ahsen, Christina Schlumbohm, Eberhard Wieland, Michael Oellerich, Victor Armstrong |
Journal | Journal of medical primatology
(J Med Primatol)
Vol. 35
Issue 3
Pg. 155-64
(Jun 2006)
ISSN: 0047-2565 [Print] Denmark |
PMID | 16764674
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Complementary
- Phospholipid Hydroperoxide Glutathione Peroxidase
- Glutathione Peroxidase
- Glutathione Peroxidase GPX1
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Callithrix
(genetics, metabolism)
- Cloning, Molecular
- DNA, Complementary
(genetics)
- Evolution, Molecular
- Gene Expression
- Glutathione Peroxidase
(genetics)
- Male
- Molecular Sequence Data
- Phospholipid Hydroperoxide Glutathione Peroxidase
- Phylogeny
- Random Amplified Polymorphic DNA Technique
(veterinary)
- Sequence Alignment
- Glutathione Peroxidase GPX1
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