[Study on changes in inflammatory cytokines and the relationship to multiple organ injury in rats with aspiration lung injury after fire-arm injury].

Abstract

OBJECTIVE:

To observe the phasic variations in concentrations of pro-inflammatory and anti-inflammatory cytokines, and to explore their potential roles in the occurrence and development process of multiple organ dysfunction.

METHODS:

Fire-arm induced aspiration lung injury model was replicated. The following parameters were observed: wet/dry weight ratio of rat's lungs, contents of albumin and lactate dehydrogenase (LDH), concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-4 in bronchial alveolar lavage fluid (BALF), contents of alanine amino transferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCr), and MB isoenzyme of creatine kinase (CK-MB).

RESULTS:

The wet/dry weight ratio of rat's lungs and contents of albumin and LDH in BALF increased with the passage of time. The concentration of TNF-alpha increased immediately after injury and reached the peak value at 2 hours. The concentration of IL-6 increased obviously 4 hours after injury, and maintained a high level from 4 hours to 24 hours. The concentration of IL-4 decreased from 6 hours to 8 hours after injury. The contents of ALT, AST, BUN, SCr and CK-MB increased continually after injury.

CONCLUSION:

Cytokines are the direct mediators for multiple organ failure (MOF), and MOF is triggered by TNF-alpha and a cascade of cytokine release, with a prolonged high-expression of IL-6. The loss of control of IL-4 aggravates the development of MOF.

• Authors: Min Zhou, Bao-ming Zou, Tao Li, Chao Lu, Wei Chen, Wen-ning Yang
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 12 Pg. 732-5 (Dec 2005) ISSN: 1003-0603 [Print] China
• PMID: 16386180 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Interleukin-6
• Tumor Necrosis Factor-alpha
• Interleukin-4

Topics

• Animals
• Cytokines (metabolism)
• Disease Models, Animal
• Female
• Interleukin-4 (metabolism)
• Interleukin-6 (metabolism)
• Lung (metabolism, pathology)
• Lung Injury (etiology, metabolism)
• Male
• Multiple Organ Failure (etiology, metabolism)
• Rats
• Rats, Wistar
• Smoke Inhalation Injury (etiology, metabolism)
• Tumor Necrosis Factor-alpha (metabolism)
• Wounds, Gunshot (complications, metabolism)