Gastrin exerts pleiotropic effects on human melanoma cell biology.

Abstract

The effects of gastrin (G17) on the growth and migration factors of four human melanoma cell lines (HT-144, C32, G-361, and SKMEL-28) were investigated. The expression patterns of cholecystokinin (CCK)(A), CCK(B), and CCK(C) gastrin receptors were investigated in these cells and in seven clinical samples by means of reverse transcription polymerase chain reaction. Melanoma cells appear to express mRNA for CCK(C) receptors, but not for CCK(A) or CCK(B) receptors. Although gastrin does not significantly modify the growth characteristics of the cell lines under study, it significantly modifies their cell migration characteristics. These modifications occur at adhesion level by modifying the expression levels of alpha(v) and beta3 integrins, at motility level by modifying the organization of the actin cytoskeleton, and at invasion level by modifying the expression levels of matrix metalloproteinase 14. We recently demonstrated the presence of CCK(B) receptors in mouse endothelial cells involved in glioblastoma neoangiogenesis. Chronic in vivo administration of a selective CCK(B) receptor antagonist to mice bearing xenografts of human C32 melanoma cells significantly decreased levels of neoangiogenesis, resulting in considerable delays in the growth of these C32 xenografts. In conclusion, our study identifies the pleiotropic effects of gastrin on melanoma cell biology.

• Authors: Véronique Mathieu, Tatjana Mijatovic, Marc van Damme, Robert Kiss
• Journal: Neoplasia (New York, N.Y.) (Neoplasia) Vol. 7 Issue 10 Pg. 930-43 (Oct 2005) ISSN: 1522-8002 [Print] United States
• PMID: 16242076 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Antineoplastic Agents
• DNA Primers
• DNA, Complementary
• Gastrins
• RNA, Messenger
• RNA, Neoplasm
• Receptor, Cholecystokinin B
• Receptors, Cholecystokinin
• Dacarbazine
• Cholecystokinin
• Poly(ADP-ribose) Polymerases
• Cisplatin

Topics

• Actins (chemistry)
• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis
• Blotting, Western
• Cell Line, Tumor
• Cell Movement
• Cholecystokinin (metabolism)
• Cisplatin (pharmacology)
• Cytoskeleton (metabolism)
• DNA Primers (chemistry)
• DNA, Complementary (metabolism)
• Dacarbazine (pharmacology)
• Flow Cytometry
• Gastrins (chemistry, metabolism, physiology)
• Gene Expression Regulation, Neoplastic
• Glioblastoma (metabolism)
• Humans
• Immunohistochemistry
• Melanoma (metabolism, pathology)
• Mice
• Neoplasm Invasiveness
• Neoplasm Transplantation
• Neovascularization, Pathologic
• Oligonucleotide Array Sequence Analysis
• Poly(ADP-ribose) Polymerases (metabolism)
• RNA, Messenger (metabolism)
• RNA, Neoplasm (metabolism)
• Receptor, Cholecystokinin B (metabolism)
• Receptors, Cholecystokinin (chemistry)
• Reverse Transcriptase Polymerase Chain Reaction
• Time Factors
• Wound Healing