Abstract |
Thrombolysis with tPA for acute ischemic stroke is associated with an increased risk of intracerebral hemorrhage. We investigated the impact of thrombolysis with tPA on the blood-brain barrier in a suture occlusion model in rats. Cerebral ischemia was performed for 2 h followed by 22 h of reperfusion. Treatment groups received either saline ( A), 10 mg/kg bw rtPA (B) or "activated" rtPA (ArtPA, C, rtPA with in vitro clot contact). Blood-brain-barrier damage assessed by Evans blue extravasation as a permeability marker was significantly enhanced in basal ganglia of group C compared to groups A or B. Likewise was the upregulation of MMP-9. Interestingly, results of the rtPA and saline group showed only minor and not statistically significant differences. The results of the present study indicate a major role for thrombus-thrombolytic interaction in focal cerebral ischemia with subsequent increased BBB permeability.
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Authors | Timo Kahles, Christian Foerch, Matthias Sitzer, Michael Schroeter, Helmuth Steinmetz, Abdelhaq Rami, Tobias Neumann-Haefelin |
Journal | Vascular pharmacology
(Vascul Pharmacol)
Vol. 43
Issue 4
Pg. 254-9
(Oct 2005)
ISSN: 1537-1891 [Print] United States |
PMID | 16185938
(Publication Type: Journal Article)
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Chemical References |
- Fibrinolytic Agents
- Evans Blue
- Tissue Plasminogen Activator
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Basal Ganglia
(pathology)
- Blood-Brain Barrier
(drug effects, physiology)
- Brain
(pathology)
- Evans Blue
- Fibrinolytic Agents
(pharmacology)
- Immunohistochemistry
- Ischemic Attack, Transient
(pathology)
- Laser-Doppler Flowmetry
- Male
- Matrix Metalloproteinase 9
(biosynthesis, metabolism)
- Rats
- Rats, Wistar
- Reperfusion Injury
(physiopathology)
- Thrombosis
(pathology)
- Tissue Plasminogen Activator
(pharmacology)
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