[Expression of ornithine decarboxylase mRNA in the cortex and hippocampus of rat after transient cerebral ischemia].

Abstract

OBJECTIVE:

To detect the expression of ornithine decarboxylase (ODC) mRNA in the cortex and hippocampus of rat over different postischemic recirculation periods, and to explore the mechanism of increase in ODC activity after transient cerebral ischemia.

METHODS:

A 10-min transient cerebral ischemia was produced in anesthetized rat by ameliorated four-vessel occlusion, and the expression of ODC mRNA was measured using comparative reverse transcription-polymerase chain reaction (RT-PCR) in the cortex and hippocampus of rats sacrificed at 2, 4, 6 and 8 h after the ischemia. The variations were studied.

RESULTS:

There was no expression of ODC mRNA in the cortex and hippocampus of rats in nonischemic control groups, and there was expression of ODC mRNA in the cortex and hippocampus of rats in the ischemic experimental groups, with a significant increase between 2 h and 6 h after the ischemia (P < 0.01).

CONCLUSION:

Increased expression of ODC mRNA could contribute to the increase in ODC activity after transient cerebral ischemia.

• Authors: Xin Wan, Bing Jiang, Yun-sheng Liu, Hong-wei Liu, Guang-yong Wu, Wen-ze Li
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 29 Issue 5 Pg. 534-6 (Oct 2004) ISSN: 1672-7347 [Print] China
• PMID: 16137040 (Publication Type: English Abstract, Journal Article)

Chemical References

• RNA, Messenger
• Ornithine Decarboxylase

Topics

• Animals
• Cerebral Cortex (metabolism)
• Hippocampus (metabolism)
• Ischemic Attack, Transient (metabolism)
• Male
• Ornithine Decarboxylase (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism)