Abstract | BACKGROUND: Inflow of tumor cells to circulation is an essential step for metastasis of primary tumors. To know its state may contribute to therapeutic strategy. However, methodology to detect lung carcinoma cells floating in peripheral blood has not been established. Pulmonary surfactant protein (SP)-A, SP-C and Clara cells-10 kd protein (CC10) are specific to the lung and often expressed in primary lung carcinomas. We evaluated the worth of these gene expressions for the detection of carcinoma cells in peripheral blood. METHODS: RESULTS: The mRNA expressions of SP-A and SP-C were completely specific to NSCLC when compared to SCLC and secondary lung tumors. All of the healthy volunteers and patients with non-malignant respiratory diseases showed negative for these mRNA expressions, except for one sample. The positive rate of SP-A, SP-C and CC10 mRNA in patients with NSCLC was 33.3%, 14.1%, 3.3%, respectively. The rates of SP-A and SP-C mRNA were higher than that (11.1%) in CEA mRNA. The increased positive rate of mRNA of SP-A and SP-C was significantly dependent on the clinical stage and the existence of distant metastasis. CONCLUSION: These results demonstrate that the detection of mRNA of SP-A and SP-C would give clinicians valuable information suggesting the presence of blood-floating carcinoma cells as a step toward metastasis.
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Authors | Orie Yamamoto, Hiroki Takahashi, Michio Hirasawa, Hirofumi Chiba, Masanori Shiratori, Yoshio Kuroki, Shosaku Abe |
Journal | Respiratory medicine
(Respir Med)
Vol. 99
Issue 9
Pg. 1164-74
(Sep 2005)
ISSN: 0954-6111 [Print] England |
PMID | 16085219
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Neoplasm Proteins
- Pulmonary Surfactant-Associated Proteins
- RNA, Messenger
- RNA, Neoplasm
- SCGB1A1 protein, human
- Uteroglobin
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Topics |
- Aged
- Biomarkers, Tumor
(blood)
- Carcinoma, Non-Small-Cell Lung
(diagnosis, pathology)
- Carcinoma, Small Cell
(diagnosis)
- Diagnosis, Differential
- Female
- Gene Expression
- Humans
- Lung Neoplasms
(diagnosis, pathology, secondary)
- Male
- Middle Aged
- Neoplasm Proteins
(blood, genetics)
- Neoplasm Staging
- Neoplastic Cells, Circulating
(metabolism)
- Pulmonary Surfactant-Associated Proteins
(blood, genetics)
- RNA, Messenger
(genetics)
- RNA, Neoplasm
(genetics)
- Respiration Disorders
(diagnosis)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Uteroglobin
(blood, genetics)
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