Nitrosative stress, heme oxygenase-1 expression and airway inflammation during severe exacerbations of COPD.

Abstract

STUDY OBJECTIVES:

The aim of this study was to examine the relationship between airway inflammation, nitrosative stress, heme-oxygenase expression, and acute severe exacerbations of COPD.

DESIGN:

We measured heme oxygenase (HO)-1, inducible nitric oxide (NO) synthase expression and nitrotyrosine formation, as well as eosinophilic cationic protein, myeloperoxidase (MPO), interleukin (IL-8), and granulocyte macrophage-colony stimulating factor levels in induced sputum samples from 12 COPD patients (mean +/- SD; FEV1 40 +/- 14% predicted) at the onset of an acute severe exacerbation of COPD requiring hospital admission and 16 weeks after remission.

RESULTS:

We demonstrated increased percentages (p = 0.001) and absolute numbers (p = 0.028) of total nitrotyrosine positive (+ve) inflammatory cells (ie, polymorphonuclear cells and macrophages), increased percentages (p = 0.04) and absolute numbers (p = 0.05) of total HO-1 +ve inflammatory cells, and increased MPO (p = 0.005) and IL-8 levels (p = 0.028) during severe exacerbation compared with the stable state.

CONCLUSIONS:

Our results support the hypothesis of an involvement of inflammatory and nitrosative stress in severe COPD exacerbations. Future therapeutic strategies may aim at regulating inflammation and NO synthesis during COPD exacerbations.

• Authors: Maria Tsoumakidou, Nikolaos Tzanakis, Georgios Chrysofakis, Nikolaos M Siafakas
• Journal: Chest (Chest) Vol. 127 Issue 6 Pg. 1911-8 (Jun 2005) ISSN: 0012-3692 [Print] United States
• PMID: 15947302 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Biomarkers
• Membrane Proteins
• Peroxidase
• Nitric Oxide Synthase
• HMOX1 protein, human
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• Eosinophil Cationic Protein

Topics

• Aged
• Biomarkers (analysis)
• Bronchitis (physiopathology)
• Cohort Studies
• Disease Progression
• Eosinophil Cationic Protein (analysis, metabolism)
• Female
• Heme Oxygenase (Decyclizing) (analysis, metabolism)
• Heme Oxygenase-1
• Humans
• Immunohistochemistry
• Male
• Membrane Proteins
• Middle Aged
• Nitric Oxide Synthase (analysis, metabolism)
• Oxidative Stress (physiology)
• Peroxidase (analysis, metabolism)
• Probability
• Prognosis
• Pulmonary Disease, Chronic Obstructive (diagnosis, enzymology)
• Risk Assessment
• Sensitivity and Specificity
• Severity of Illness Index
• Sputum
• Statistics, Nonparametric