Abstract |
This study was designed to determine the role of interleukin (IL)-1 in the inflammatory response against experimentally induced pneumonia caused by Klebsiella pneumoniae. The host immune responses of IL-1 gene knockout (IL-1 KO) mice and immunocompetent wild-type (WT) mice were compared after pulmonary infection with K. pneumoniae. There were no significant differences between the survival rates and viable bacterial counts in lungs and blood of IL-1 KO and WT mice after pulmonary infections under different conditions. Histopathological analysis showed a similar inflammatory response in both groups of mice. However, in the early stage of infection, the level of tumour necrosis factor alpha ( TNF-alpha) in homogenized lungs of IL-1 KO mice was significantly higher than in WT mice. To determine the role of endogenous TNF-alpha in the recovery of the defence mechanism in IL-1 KO mice, mice were treated with an anti- TNF-alpha mAb before infection with K. pneumoniae. The results revealed a significantly lower survival rate of anti- TNF-alpha mAb-treated IL-1 KO mice than BSA-treated IL-1 KO mice. The data suggest that compensatory production of TNF-alpha in IL-1 KO mice contributes to the host defence against K. pneumoniae infection.
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Authors | Masaaki Tanabe, Tetsuya Matsumoto, Kazutoshi Shibuya, Kazuhiro Tateda, Shuichi Miyazaki, Akio Nakane, Yoichiro Iwakura, Keizo Yamaguchi |
Journal | Journal of medical microbiology
(J Med Microbiol)
Vol. 54
Issue Pt 1
Pg. 7-13
(Jan 2005)
ISSN: 0022-2615 [Print] England |
PMID | 15591249
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-1
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Blood
(microbiology)
- Colony Count, Microbial
- Disease Models, Animal
- Interleukin-1
(genetics, immunology)
- Klebsiella Infections
(immunology)
- Klebsiella pneumoniae
(immunology, isolation & purification)
- Lung
(chemistry, microbiology, pathology)
- Male
- Mice
- Mice, Knockout
- Pneumonia, Bacterial
(immunology, microbiology)
- Survival Analysis
- Tumor Necrosis Factor-alpha
(analysis, immunology)
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