Abstract | BACKGROUND: OBJECTIVE: METHODS:
NGF-tg mice, which overexpress NGF in Clara cells of the airways, were compared with wild-type (wt) littermates regarding their ability to mount IgE-related airway inflammatory responses. Mice were sensitized intraperitoneally to ovalbumin (OVA) and locally challenged via the airways according to established protocols. RESULTS:
NGF-tg mice displayed enhanced levels of OVA-specific IgE antibody titres after repeated OVA aerosol exposure. In the airways, increased numbers of eosinophils were detected. These results were confirmed to be NGF specific, because similar results were obtained following local application of NGF into the airways of wt mice. The effect of NGF was partly mediated via neuropeptides, as treatment of OVA-sensitized NGF-tg mice with the dual neurokinin (NK) receptor NK-1/ NK-2 antagonist partly prevented enhanced airway inflammation. CONCLUSION: The present data indicate an important functional role of NGF in allergic airway inflammation and point to an involvement of tachykinins as mediators of NGF effects.
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Authors | D Quarcoo, O Schulte-Herbrüggen, M Lommatzsch, K Schierhorn, G W Hoyle, H Renz, A Braun |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 34
Issue 7
Pg. 1146-51
(Jul 2004)
ISSN: 0954-7894 [Print] England |
PMID | 15248864
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-5
- Nerve Growth Factors
- Peptides, Cyclic
- Receptors, Tachykinin
- Tachykinins
- FK 224
- Ovalbumin
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Topics |
- Animals
- Asthma
(immunology)
- Bronchial Hyperreactivity
- Eosinophils
(immunology)
- Humans
- Interleukin-5
(blood)
- Leukocyte Count
- Lung
(immunology)
- Mice
- Mice, Transgenic
- Models, Animal
- Nerve Growth Factors
(blood, metabolism)
- Ovalbumin
- Peptides, Cyclic
(pharmacology)
- Receptors, Tachykinin
(antagonists & inhibitors)
- Tachykinins
(metabolism)
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