Cytoglobin expression is upregulated in all tissues upon hypoxia: an in vitro and in vivo study by quantitative real-time PCR.

Abstract

The vertebrate globin family has been extended with two members: neuroglobin and cytoglobin. We here investigate the changes of expression levels upon hypoxia of cytoglobin in parallel with neuroglobin, in vivo and in vitro, by using real-time quantitative PCR. Our data prove that cytoglobin is upregulated upon hypoxia in all tissues. The mechanism of induction of cytoglobin is regulated by the hypoxia-inducible factor 1, a posttranscriptionally regulated transcription factor controlling several hypoxia-inducible genes. The latter is argumented by: (1) cytoglobin is significantly upregulated upon hypoxia and this is dependent on the tissue and severity of hypoxia; (2) the regulation of cytoglobin expression in HIF-1 (+/-) knockout mice is affected; (3) the variations of the expression regulation are in the same manner as seen in the expression of our control gene VEGF, that is proven to be regulated by the HIF-1-pathway; and (4) cytoglobin promoter region contains HRE sites.

• Authors: E Fordel, E Geuens, S Dewilde, P Rottiers, P Carmeliet, J Grooten, L Moens
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 319 Issue 2 Pg. 342-8 (Jun 25 2004) ISSN: 0006-291X [Print] United States
• PMID: 15178412 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Globins

Topics

• Animals
• Globins (genetics, physiology)
• Hypoxia (physiopathology)
• In Vitro Techniques
• Mice
• Mice, Knockout
• Polymerase Chain Reaction
• Up-Regulation