Coexpression of cholecystokinin-B/gastrin receptor and gastrin gene in human gastric tissues and gastric cancer cell line.

Abstract

AIM:

To compare the expression patterns of cholecystokinin-B (CCK-B)/gastrin receptor genes in matched human gastric carcinoma and adjacent non-neoplastic mucosa of patients with gastric cancer, inflammatory gastric mucosa from patients with gastritis, normal stomachs from 2 autopsied patients and a gastric carcinoma cell line (SGC-7901), and to explore their relationship with progression to malignancy of human gastric carcinomas.

METHODS:

RT-PCR and sequencing were employed to detect the mRNA expression levels of CCK-B receptor and gastrin gene in specimens from 30 patients with gastric carcinoma and healthy bordering non-cancerous mucosa, 10 gastritis patients and normal stomachs from 2 autopsied patients as well as SGC-7901. The results were semi-quantified by normalizing it to the mRNA level of beta-actin gene using Lab Image software. The sequences were analyzed by BLAST program.

RESULTS:

CCK-B receptor transcripts were detected in all of human gastric tissues in this study, including normal, inflammatory and malignant tissues and SGC-7901. However, the expression levels of CCK-B receptor in normal gastric tissues were higher than those in other groups (P<0.05), and its expressions did not correlate with the differentiation and metastasis of gastric cancer (P>0.05). On the other hand, gastrin mRNA was detected in SGC-7901 and in specimens obtained from gastric cancer patients (22/30) but not in other gastric tissues, and its expression was highly correlated with the metastases of gastric cancer (P<0.05).

CONCLUSION:

Human gastric carcinomas and gastric cancer cell line SGC-7901 cells coexpress CCK-B receptor and gastrin mRNA. Gastrin/CCK-B receptor autocrine or paracrine pathway may possibly play an important role in the progression of gastric cancer.

• Authors: Jian-Jiang Zhou, Man-Ling Chen, Qun-Zhou Zhang, Jian-Kun Hu, Wen-Ling Wang
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 10 Issue 6 Pg. 791-4 (Mar 15 2004) ISSN: 1007-9327 [Print] United States
• PMID: 15040018 (Publication Type: Journal Article)

Chemical References

• Gastrins
• Receptor, Cholecystokinin B

Topics

• Base Sequence
• Cell Line, Tumor
• Gastric Mucosa (metabolism)
• Gastrins (genetics)
• Gene Expression
• Humans
• Molecular Sequence Data
• Receptor, Cholecystokinin B (metabolism)
• Stomach Neoplasms (metabolism)