Abstract | BACKGROUND/PURPOSE: METHODS: Genomic DNA was extracted from archival paraffin-embedded lung tissue from 13 newborns with CDH complicated by PPH and from 9 controls. Genotyping for the I/D-ACE, the M235 T-AGT, and the A1166 C-ATIR gene polymorphisms were determined by a polymerase chain reaction-based method with appropriate restriction digest when required. RESULTS: In controls, ACE genotype distribution of DD, ID, and II was 11%, 33%, and 55%, respectively, whereas in CDH it was 70%, 15%, and 15%, respectively. The ACE-DD genotype was significantly higher in CDH compared with controls (P <.05). In CDH samples, the prevalence of AGT-MM genotype was lower (8% v. 33%; P <.05), whereas the AGT-TT genotype was higher (61% v. 22%; P <.05) compared with controls. There were no differences in allele frequencies of AT1R between CDH patients and controls. CONCLUSIONS:
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Authors | Valeria Solari, Prem Puri |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 39
Issue 3
Pg. 302-6; discussion 302-6
(Mar 2004)
ISSN: 1531-5037 [Electronic] United States |
PMID | 15017542
(Publication Type: Journal Article)
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Chemical References |
- Receptors, Angiotensin
- Angiotensinogen
- Angiotensin II
- Peptidyl-Dipeptidase A
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Topics |
- Alleles
- Angiotensin II
- Angiotensinogen
(genetics)
- Case-Control Studies
- Gene Frequency
- Hernia, Diaphragmatic
(complications, genetics)
- Hernias, Diaphragmatic, Congenital
- Humans
- Hypertension, Pulmonary
(complications, congenital, genetics)
- Infant, Newborn
- Peptidyl-Dipeptidase A
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Receptors, Angiotensin
(genetics)
- Renin-Angiotensin System
(genetics)
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