Abstract | OBJECTIVE: METHODOLOGY: RESULTS: We found that PGIS mRNA expression was suppressed by high concentrations of TNF-alpha and MIF, while COX-2 mRNA was induced by all three cytokines tested. IL-1beta increased PGI2 production in a dose-dependent manner. TNF-alpha and MIF also increased PGI2 production, but to a far lesser degree at high concentrations. TNF-alpha paradoxically decreased PGI2 production at a low concentration. CONCLUSIONS: These results suggest that TNF-alpha and MIF are potentially antagonistic to the action of PGI2 in rat PASMCs via down-regulation of PGIS mRNA. Simultaneous induction of COX-2 mRNA may further counteract the action of PGI2 by increasing the levels of eicosanoids other than PGI2.
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Authors | Akihide Itoh, Jun Nishihira, Hironi Makita, Kenji Miyamoto, Etsuro Yamaguchi, Masaharu Nishimura |
Journal | Respirology (Carlton, Vic.)
(Respirology)
Vol. 8
Issue 4
Pg. 467-72
(Dec 2003)
ISSN: 1323-7799 [Print] Australia |
PMID | 14629650
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-1
- Isoenzymes
- Macrophage Migration-Inhibitory Factors
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- 6-Ketoprostaglandin F1 alpha
- Cytochrome P-450 Enzyme System
- Epoprostenol
- Cyclooxygenase 2
- Prostaglandin-Endoperoxide Synthases
- Intramolecular Oxidoreductases
- prostacyclin synthetase
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Topics |
- 6-Ketoprostaglandin F1 alpha
(metabolism)
- Analysis of Variance
- Animals
- Cells, Cultured
- Cyclooxygenase 2
- Cytochrome P-450 Enzyme System
(metabolism)
- Epoprostenol
(biosynthesis, immunology)
- Interleukin-1
(metabolism)
- Intramolecular Oxidoreductases
(metabolism)
- Isoenzymes
(metabolism)
- Macrophage Migration-Inhibitory Factors
(metabolism)
- Muscle, Smooth, Vascular
(immunology, metabolism)
- Myocytes, Smooth Muscle
(immunology, metabolism)
- Prostaglandin-Endoperoxide Synthases
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Tumor Necrosis Factor-alpha
(metabolism)
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