Abstract |
The present study aimed at understanding the effects of arachidonic acid peroxides on neuronal cell death using the mouse neuroblastoma cell line, Neuro-2A cells. Arachidonic acid peroxides were produced by ultraviolet (UV) radiation. UV-radiated arachidonic acid significantly reduced Neuro-2A cell viability at concentrations of more than 0.1 muM, with being more potential than non-radiated arachidonic acid. Nuclei of Neuro-2A cells killed with UV-radiated arachidonic acid were reactive to Hoechst 33342, a marker of apoptosis, and the effect was much greater than that achieved with non-radiated arachidonic acid. UV-radiated arachidonic acid persistently increased intracellular Ca(2+) concentrations and dissipated mitochondrial membrane potential in Neuro-2A cells. UV-radiated arachidonic acid-induced Neuro-2A cell death, whereas it was not affected by a pancaspase inhibitor or a caspase-3 inhibitor, was significantly inhibited by an inhibitor of caspase-1, -8, or -9. The results of the present study suggest that arachidonic acid peroxides induce apoptotic neuronal cell death in association with intracellular Ca(2+) rise and mitochondrial damage, in part via a caspase-dependent pathway regardless of caspase-3.
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Authors | Masaru Saitoh, Kaoru Nagai, Takahiro Yaguchi, Yoshiko Fujikawa, Keiko Ikejiri, Satoshi Yamamoto, Kazuhiko Nakagawa, Takehira Yamamura, Tomoyuki Nishizaki |
Journal | Brain research
(Brain Res)
Vol. 991
Issue 1-2
Pg. 187-94
(Nov 21 2003)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 14575891
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Enzyme Inhibitors
- Lipid Peroxides
- Arachidonic Acid
- Casp3 protein, mouse
- Caspase 3
- Caspases
- Calcium
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Topics |
- Animals
- Apoptosis
(drug effects, physiology)
- Arachidonic Acid
(metabolism, radiation effects)
- Calcium
(metabolism)
- Caspase 3
- Caspases
(metabolism)
- Cell Line, Tumor
- Enzyme Activation
- Enzyme Inhibitors
(pharmacology)
- Fluorescent Antibody Technique
- Intracellular Fluid
(drug effects, metabolism)
- Lipid Peroxides
(pharmacology)
- Membrane Potentials
(drug effects, physiology)
- Mice
- Mitochondria
(drug effects, pathology)
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