Abstract | BACKGROUND & OBJECTIVE: METHODS: Sixty-six patients of breast cancer with clinical features and survival data were enrolled. The mRNA expression of TIMP-2, MMP-2 were determined using in situ hybridization. The protein expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 were determined using immunohistochemistry. The results were analyzed using chi-square test, Kaplan-Meier method, and Cox multivariate regression analysis. RESULTS: The positive expression rates of TIMP-2 mRNA, MMP-2 mRNA and MMP-2, MMP-9, TIMP-1, and TIMP-2 protein were 66.7% (44/66), 65.2% (43/66) and 71.2% (47/66), 68.2% (45/66), 40.9% (27/66), 69.7% (46/66), respectively. The expression of MMP-2 protein had positive correlation with those of MMP-2 mRNA and MMP-9 protein(P< 0.01). The expression of TIMP-2 mRNA had positive correlation with that of TIMP-2 protein. Negative correlation between expression of TIMP-1 protein and MMP-9 protein was found (P< 0.01). Overexpression of MMP-2 and MMP-9 protein were higher in breast cancers with lymph node metastases than those without lymph node metastases, whereas TIMP-2 mRNA and TIMP-1 protein expression were lower in breast cancers with lymph node metastases than those without lymph node metastases (P< 0.05). MMP-2 mRNA and MMP-9 protein were positively associated with the tumor size and shortened survival time(P< 0.05, P< 0.01). Increased expression of MMP-9 protein was correlated with high TNM classification(P< 0.01). The patients of menopause and ER negative expression had higher expression of MMP-2 mRNA (P< 0.05). Kaplan-Meier analysis showed that MMP-2 mRNA, MMP-2 and MMP-9 proteins were linked with unfavorable prognosis(P< 0.01,P< 0.05). CONCLUSION: Overexpression of MMP-2, MMP-9 and expression imbalance between MMP-9 and TIMP-1 protein might play critical roles in degradation of extracellular matrix to enhance the invasive and metastatic capacity of breast cancer. MMP-2 protein might be applied as an independent prognostic indicator for primary breast cancer.
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Authors | Song-Qing Fan, Qi-You Wei, Mei-Rong Li, Li-Qun Zhang, Qing-Chun Liang |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 22
Issue 9
Pg. 968-73
(Sep 2003)
China |
PMID | 12969531
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- RNA, Messenger
- Tissue Inhibitor of Metalloproteinase-1
- Tissue Inhibitor of Metalloproteinase-2
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
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Topics |
- Adult
- Aged
- Breast Neoplasms
(enzymology, mortality, pathology)
- Female
- Humans
- In Situ Hybridization
- Matrix Metalloproteinase 2
(genetics)
- Matrix Metalloproteinase 9
(genetics)
- Middle Aged
- Prognosis
- RNA, Messenger
(analysis)
- Tissue Inhibitor of Metalloproteinase-1
(genetics)
- Tissue Inhibitor of Metalloproteinase-2
(genetics)
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