Abstract | AIMS/HYPOTHESIS: METHODS: Ventricular myocytes were cultured for 24 h with either normal (N, 5.5 mmol/l) or high (25.5 mmol/ l) glucose, with or without the nitric oxide scavengers haemoglobin (100 nmol/l), PTIO (100 micromol/l), the NOS inhibitor L-NMMA (100 micromol/l), superoxide dismutase (SOD, 500 U/ml), the ONOO(-) scavengers urate (100 micromol/l), MnTABP (100 micromol/l), BH(4) (10 micromol/l) and its inactive analogue NH(4) (10 micromol/l), and the GTP cyclohydrolase I inhibitor DAHP (1 mmol/l). Myocyte mechanics, NOS protein expression and activity were evaluated. RESULTS: High glucose myocytes showed reduced peak shortening, decreased maximal velocity of shortening/relengthening (+/- dL/dt), prolonged relengthening (TR(90)) and normal shortening duration (TPS) associated with reduced cytosolic Ca(2+) rise compared to normal myocytes. The high glucose-induced abnormalities were abrogated or attenuated by urate, MnTBAP, L-NMMA, BH(4), and SOD, whereas unaffected by haemoglobin, PTIO and NH(4). L-NMMA reduced peak shortening while PTIO and DAHP depressed +/- dL/dt and prolonged TPS or TR(90) in normal myocytes. High glucose increased NOS activity, protein expression of eNOS but not iNOS, which were attenuated by L-NMMA and BH(4), respectively. CONCLUSION/INTERPRETATION: These results suggested that NOS cofactor, NO and ONOO(-) play a role in glucose-induced cardiomyocyte contractile dysfunction and in the pathogenesis of diabetic cardiomyopathy.
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Authors | L B Esberg, J Ren |
Journal | Diabetologia
(Diabetologia)
Vol. 46
Issue 10
Pg. 1419-27
(Oct 2003)
ISSN: 0012-186X [Print] Germany |
PMID | 12898015
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Enzyme Inhibitors
- Free Radical Scavengers
- Peroxynitrous Acid
- Biopterins
- omega-N-Methylarginine
- Nitric Oxide
- Nitric Oxide Synthase
- Superoxide Dismutase
- sapropterin
- Glucose
- Calcium
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Topics |
- Animals
- Biopterins
(analogs & derivatives, metabolism)
- Calcium
(metabolism)
- Cells, Cultured
- Enzyme Inhibitors
(pharmacology)
- Free Radical Scavengers
(pharmacology)
- Glucose
(poisoning)
- Intracellular Membranes
(metabolism)
- Male
- Myocardial Contraction
(drug effects)
- Myocytes, Cardiac
(drug effects, metabolism)
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase
(antagonists & inhibitors, metabolism)
- Peroxynitrous Acid
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Superoxide Dismutase
(pharmacology)
- Ventricular Dysfunction
(metabolism, physiopathology)
- omega-N-Methylarginine
(pharmacology)
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