The influence of macrophage inflammatory protein-1alpha on protective immunity mediated by antiviral cytotoxic T cells.

Abstract

Macrophage inflammatory protein 1alpha (MIP-1alpha), a member of the CC-chemokine subfamily, is known to induce chemotaxis of a variety of cell types in vivo. Although the role of MIP-1alpha in inflammatory responses generated following primary infection of mice with many different pathogens has been characterized, the influence of this chemokine on the generation of antigen-specific T-cell responses in vivo is less well understood. This is important, as virus-specific CD8+ T lymphocytes (CTL) play a crucial role in defence against viral infections, both acutely and in the long term. In this study, we compared the ability of wild-type and MIP-1alpha-deficient (MIP-1alpha-/-) mice to mount CTL responses specific for the immunodominant epitope derived from influenza nucleoprotein (NP366-374). Influenza-specific CTL responses were compared with respect to frequency, cytotoxic activity and ability to clear subsequent infections with recombinant vaccinia viruses expressing the influenza NP. The results indicate that antiviral CTL generated in MIP-1alpha-/- mice are slightly impaired in their ability to protect against a subsequent infection. However, impaired in vivo CTL-mediated antiviral protection was found to be associated with reduced cytotoxicity rather than with a failure of the CTL to migrate to peripheral sites of infection.

• Authors: Emma Jones, David A Price, Michaela Dahm-Vicker, Vincenzo Cerundolo, Paul Klenerman, Awen Gallimore
• Journal: Immunology (Immunology) Vol. 109 Issue 1 Pg. 68-75 (May 2003) ISSN: 0019-2805 [Print] England
• PMID: 12709019 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Chemokine CCL3
• Chemokine CCL4
• Macrophage Inflammatory Proteins
• Peptide Fragments
• Viral Core Proteins
• nucleoprotein (366-374), influenza virus

Topics

• Animals
• Antigens, Viral (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Chemokine CCL3
• Chemokine CCL4
• Chemotaxis, Leukocyte (immunology)
• Cytotoxicity, Immunologic
• Female
• Immunity, Cellular
• Immunologic Memory
• Macrophage Inflammatory Proteins (deficiency, immunology)
• Mice
• Mice, Inbred C57BL
• Orthomyxoviridae Infections (immunology, prevention & control)
• Peptide Fragments (immunology)
• T-Lymphocytes, Cytotoxic (immunology)
• Viral Core Proteins (immunology)