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Expression of tumor necrosis factor-alpha in regenerating muscle fibers in inflammatory and non-inflammatory myopathies.

Abstract
The expression level of tumor necrosis factor (TNF)-alpha is elevated in idiopathic inflammatory myopathies and Duchenne muscular dystrophy (DMD), but the precise role of TNF-alpha is unknown. To elucidate the possible role of TNF-alpha, we investigated the expression of TNF-alpha and its receptor in polymyositis (PM), dermatomyositis (DM), and DMD using in situ hybridization (ISH) and immunohistochemistry. We showed that TNF-alpha mRNA and protein were present in muscle fibers. TNF-alpha-positive fibers were observed in all cases of PM, DM and DMD, but were rare or absent in neurogenic disorders and normal controls. The proportion of TNF-alpha-positive fiber showed a significant positive correlation with the proportion of regenerating fibers that were positive for the developmental form of myosin heavy chain (MHC-d). The number of TNF receptor-positive fibers was small. Some muscle fibers expressed both TNF-alpha and its receptor simultaneously. Our results indicate that TNF-alpha is produced and expressed by muscle fibers and associated with muscle regeneration.
AuthorsSatoshi Kuru, Akira Inukai, Takashi Kato, Yideng Liang, Seigo Kimura, Gen Sobue
JournalActa neuropathologica (Acta Neuropathol) Vol. 105 Issue 3 Pg. 217-24 (Mar 2003) ISSN: 0001-6322 [Print] Germany
PMID12557007 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor
  • Tumor Necrosis Factor-alpha
  • Myosin Heavy Chains
Topics
  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Dermatomyositis (metabolism, pathology)
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Middle Aged
  • Muscle Fibers, Skeletal (pathology)
  • Muscle, Skeletal (pathology, physiology)
  • Muscular Diseases (metabolism, pathology)
  • Muscular Dystrophy, Duchenne (metabolism, pathology)
  • Myosin Heavy Chains (metabolism)
  • Polymyositis (metabolism, pathology)
  • RNA, Messenger (analysis)
  • Receptors, Tumor Necrosis Factor (biosynthesis)
  • Regeneration
  • Tumor Necrosis Factor-alpha (biosynthesis)

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