The CCR3 receptor is involved in eosinophil differentiation and is up-regulated by Th2 cytokines in CD34+ progenitor cells.

Abstract	The involvement of chemokines in eosinophil recruitment during inflammation and allergic reactions is well established. However, a functional role for chemokines in eosinophil differentiation has not been investigated. Using in situ RT-PCR, immunostaining, and flow cytometric analysis, we report that human CD34+ cord blood progenitor cells contain CCR3 mRNA and protein. Activation of CD34+ progenitor cells under conditions that promote Th2 type differentiation up-regulated surface expression of the CCR3. In contrast, activation with IL-12 and IFN-gamma resulted in a significant decrease in the expression of CCR3. Eotaxin induced Ca2+ mobilization in CD34+ progenitor cells, which could explain the in vitro and in vivo chemotactic responsiveness to eotaxin. We also found that eotaxin induced the differentiation of eosinophils from cord blood CD34+ progenitor cells. The largest number of mature eosinophils was found in cultures containing eotaxin and IL-5. The addition of neutralizing anti-IL-3, anti-IL-5, and anti-GM-CSF Abs to culture medium demonstrated that the differentiation of eosinophils in the presence of eotaxin was IL-3-, IL-5-, and GM-CSF-independent. These results could explain how CD34+ progenitor cells accumulate and persist in the airways and peripheral blood of patients with asthma and highlight an alternative mechanism by which blood and tissue eosinophilia might occur in the absence of IL-5.
Authors	Bouchaib Lamkhioued, Soussi Gounni Abdelilah, Qutayba Hamid, Nabil Mansour, Guy Delespesse, Paolo M Renzi
Journal	Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 170 Issue 1 Pg. 537-47 (Jan 01 2003) ISSN: 0022-1767 [Print] United States
PMID	12496441 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, CD34 CCL11 protein, human CCR3 protein, human Ccl11 protein, mouse Ccr3 protein, mouse Chemokine CCL11 Chemokines, CC Chemotactic Factors, Eosinophil Cytokines Drug Combinations Growth Inhibitors Immune Sera Interleukin-3 Interleukin-5 RNA, Messenger Receptors, CCR3 Receptors, Chemokine Granulocyte-Macrophage Colony-Stimulating Factor
Topics	Animals Antigens, CD34 (biosynthesis) Calcium Signaling (immunology) Cell Differentiation (immunology) Cell Membrane (immunology, metabolism) Cells, Cultured Chemokine CCL11 Chemokines, CC (metabolism, pharmacology) Chemotactic Factors, Eosinophil (metabolism, pharmacology) Chemotaxis, Leukocyte (immunology) Cytokines (physiology) Dose-Response Relationship, Immunologic Drug Combinations Eosinophils (cytology, immunology, metabolism) Fetal Blood (cytology, immunology) Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor (immunology) Growth Inhibitors (pharmacology) Hematopoietic Stem Cells (cytology, immunology, metabolism) Humans Immune Sera (pharmacology) Interleukin-3 (immunology) Interleukin-5 (immunology) Kinetics Male Mice Mice, Inbred BALB C RNA, Messenger (biosynthesis) Receptors, CCR3 Receptors, Chemokine (biosynthesis, genetics, physiology) Th2 Cells (immunology, metabolism) Time Factors Up-Regulation (immunology)

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