Cyanidin 3-O-beta-D-glucoside attenuates the hepatic ischemia-reperfusion injury through a decrease in the neutrophil chemoattractant production in rats.

Abstract

We have shown that the orally administered cyanidin 3-O-beta-D-glucoside (C3G) attenuates the hepatic ischemia-reperfusion (I/R) injury, which was used as a model for oxidative stress through a decrease in neutrophil chemoattractant production in rats. The rats were subjected to hepatic I/R at 30 min after the administration of C3G (0.9 mmol/kg body weight) or vehicle. I/R treatment resulted in the elevation of oxidative stress marker [liver thiobarbituric acid-reactive substance, Nepsilon-(hexanonyl) lysine and dityrosine] levels in the liver and of the serum activities of marker enzymes for liver injury. The administration of C3G significantly suppressed these elevations, which had been caused by hepatic I/R. Liver myeloperoxidase activity, a useful marker for neutrophil infiltration into tissues, and the plasma and liver concentration of cytokine-induced neutrophil chemoattractant-1 (CINC-1), which has a potent chemotactic activity, were markedly elevated in the control group after hepatic I/R. However, these elevations were significantly suppressed in the C3G group. C3G and its metabolites in the plasma and liver were detected in the C3G group after hepatic I/R. These results suggest that the absorbed C3G and/or its metabolites can act as antioxidants in the blood and liver and scavenge the reactive oxygen species, and brought on a decrease in neutrophil infiltration into the liver through the suppression of CINC-1 production and the tissue damage caused by neutrophils after I/R is attenuated.

• Authors: Takanori Tsuda, Fumihiko Horio, Yoji Kato, Toshihiko Osawa
• Journal: Journal of nutritional science and vitaminology (J Nutr Sci Vitaminol (Tokyo)) Vol. 48 Issue 2 Pg. 134-41 (Apr 2002) ISSN: 0301-4800 [Print] Japan
• PMID: 12171434 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthocyanins
• Antioxidants
• Biomarkers
• Chemotactic Factors
• Glucosides
• Thiobarbituric Acid Reactive Substances
• cyanidin-3-O-beta-glucopyranoside
• Peroxidase

Topics

• Animals
• Anthocyanins (chemistry, pharmacology)
• Antioxidants (pharmacology)
• Biomarkers
• Chemotactic Factors (biosynthesis)
• Glucosides (chemistry, pharmacology)
• Liver (blood supply, enzymology)
• Male
• Neutrophils (metabolism)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)
• Thiobarbituric Acid Reactive Substances (analysis)