Abstract | UNLABELLED: METHODS: Partial right- knee arthroplasty was performed on 13 New Zealand White female rabbits, with a tibial silicone-elastomer implant fitting into the intramedullary canal of the tibia. After the surgical wound was closed, 10(7) cfu of a clinical strain of methicillin-susceptible Staphylococcus aureus were injected into the joint in G1 rabbits. G2 rabbits were injected with sterile saline. No antibiotic therapy was given to the animals. (99m)Tc-ciprofloxacin planar imaging was performed on days 5, 12, and 19 after surgery, and after 3 mo in 1 uninfected rabbit. Images were obtained 1, 4, and 24 h after injection (147 +/- 13 MBq). RESULTS: In G1, increased right knee (99m)Tc-ciprofloxacin uptake was observed in 3 of 5 rabbits on day 5, and in all cases on days 12 and 19. Killing of the animals revealed purulent arthritis, osteitis, and tibial myelitis. In G2, significant right-knee uptake was found on days 12 and 19 in 5 of 6 rabbits, and after 3 mo in 1; all sets of images were negative in 1 animal. Bacteriologic studies after the animals were killed were negative in G2. Mean right/left knee uptake ratios on day 19 (4-h images) were 1.8 +/- 0.4 in G1 versus 1.4 +/- 0.3 in G2 (not significant). Late images did not discriminate between infected and uninfected arthroplasty. CONCLUSION: Results of (99m)Tc-ciprofloxacin imaging in rabbits with infected/uninfected knee prosthesis suggest good sensitivity but lack of specificity for the detection of S. aureus infection.
|
Authors | Laure Sarda, Azzam Saleh-Mghir, Can Peker, Alain Meulemans, Anne-Claude Crémieux, Dominique Le Guludec |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 43
Issue 2
Pg. 239-45
(Feb 2002)
ISSN: 0161-5505 [Print] United States |
PMID | 11850491
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Infective Agents
- Ciprofloxacin
- Technetium
|
Topics |
- Animals
- Anti-Infective Agents
(pharmacokinetics)
- Autoradiography
- Ciprofloxacin
(pharmacokinetics)
- Female
- Knee Joint
(diagnostic imaging)
- Knee Prosthesis
(adverse effects)
- Prosthesis-Related Infections
(diagnostic imaging)
- Rabbits
- Radionuclide Imaging
- Staphylococcal Infections
(diagnostic imaging)
- Technetium
(pharmacokinetics)
- Tissue Distribution
|