Abstract |
The study was designed to determine the role of interferon (IFN)-gamma in inflammatory responses against experimentally induced pneumonia caused by Klebsiella pneumoniae. The host immunological responses in IFN-gamma gene knockout (IFN-gamma(-/-)) mice and immunocompetent control mice were compared. K. pneumoniae strain T-113 was inoculated intranasally into anaesthetised mice to induce pneumonia. Infected control mice survived significantly longer than infected IFN-gamma(-/-) mice. Viable bacterial counts in lungs and blood abruptly increased in IFN-gamma(-/-) mice; in contrast, a gradual decrease in the number of bacteria was noted in control mice. During the early stages of infection, the concentrations of interleukin (IL)-1beta and IL-6 in broncho-alveolar lavage fluid and IL-1beta in serum of IFN-gamma(-/-) mice were significantly lower than in control mice. During the late stage of infection, serum IL-6 level in IFN-gamma(-/-) mice was significantly higher than in control mice. These results suggest that the defective immunological host response, including inflammatory cytokine production caused by deficiency of IFN-gamma, is one of the mechanisms that allow the progression of pulmonary infection to systemic septicaemia.
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Authors | Kanako Yoshida, Tetsuya Matsumoto, Kazuhiro Tateda, Kou Uchida, Shiro Tsujimoto, Yoichiro Iwakura, Keizo Yamaguchi |
Journal | Journal of medical microbiology
(J Med Microbiol)
Vol. 50
Issue 11
Pg. 959-964
(Nov 2001)
ISSN: 0022-2615 [Print] England |
PMID | 11699592
(Publication Type: Journal Article)
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Chemical References |
- Cytokines
- Interferon-gamma
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Topics |
- Animals
- Bronchoalveolar Lavage Fluid
(immunology)
- Colony Count, Microbial
- Cytokines
(analysis, blood)
- Disease Models, Animal
- Humans
- Interferon-gamma
(immunology, metabolism)
- Kinetics
- Klebsiella Infections
(immunology, microbiology, mortality)
- Klebsiella pneumoniae
(immunology, isolation & purification)
- Lung
(microbiology, pathology)
- Mice
- Mice, Knockout
- Phagocytes
(immunology)
- Pneumonia, Bacterial
(immunology, microbiology, mortality)
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