Reduced hyperalgesia induced by nerve injury, but not by inflammation in mice lacking protein kinase C gamma isoform.

Abstract

Protein kinase C is one of protein kinases which might be involved in the nerve injury- or inflammation-induced hyperalgesia. The present study was designed to investigate the hyperalgesia with thermal paw-withdrawal test induced by sciatic nerve ligation or by intraplantar injection of a complete Freund's adjuvant solution in protein kinase C gamma knockout and its wild-type mice. Either sciatic nerve ligation or intraplantar injection of a complete Freund's adjuvant caused a marked decrease of the paw-withdrawal latency only on the ipsilateral, but not on the contralateral side of the paw in wild-type mice. This ipsilateral hyperalgesia induced by sciatic nerve ligation was significantly attenuated in protein kinase C gamma knockout mice. On the other hand, the ipsilateral hyperalgesia induced by complete Freund's adjuvant remained about the same in protein kinase C gamma knockout mice as in wild-type mice. The results indicate that protein kinase C gamma is involved in the development of the thermal hyperalgesia induced by nerve ligation, but not by complete Freund's adjuvant-induced inflammation.

• Authors: M Ohsawa, M Narita, H Mizoguchi, E Cheng, L F Tseng
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 429 Issue 1-3 Pg. 157-60 (Oct 19 2001) ISSN: 0014-2999 [Print] Netherlands
• PMID: 11698038 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Isoenzymes
• Freund's Adjuvant
• protein kinase C gamma
• Protein Kinase C

Topics

• Animals
• Disease Models, Animal
• Freund's Adjuvant
• Hyperalgesia (enzymology, genetics, prevention & control)
• Inflammation (chemically induced, enzymology, genetics)
• Isoenzymes (deficiency, genetics)
• Ligation
• Male
• Mice
• Mice, Knockout
• Protein Kinase C (deficiency, genetics)
• Sciatic Nerve (enzymology, pathology, physiopathology)