A novel amplicon at 9p23 - 24 in squamous cell carcinoma of the esophagus that lies proximal to GASC1 and harbors NFIB.

Abstract

The non-random amplification of DNA at 9p23 - 24 observed in various types of human cancers, including esophageal squamous cell carcinomas (ESCs), may reflect the locations of important tumor-associated genes. Our previous studies using ESC cell lines defined an amplicon in this region and identified a novel gene, GASC1, as a target of the amplification. Since different regions within the same chromosome arm are often involved in amplification in a syntenic or non-syntenic manner, we characterized the amplicon at 9p23 - 24 in 35 ESC cell lines (29 KYSE series and 6 YES series), and examined possible involvement of non-syntenic amplifications at 9p23 - 24 in 32 primary ESCs. Our results clearly indicated that two target regions for DNA amplification exist at 9p23 - 24; the major amplicon contains GASC1, and the minor one harbors a transcription factor, NFIB, centromeric to the GASC1 locus.

• Authors: Z Q Yang, I Imoto, A Pimkhaokham, Y Shimada, K Sasaki, M Oka, J Inazawa
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 92 Issue 4 Pg. 423-8 (Apr 2001) ISSN: 0910-5050 [Print] Japan
• PMID: 11346465 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• KDM4C protein, human
• NFI Transcription Factors
• NFIB protein, human
• Neoplasm Proteins
• Proteins
• Transcription Factors
• Jumonji Domain-Containing Histone Demethylases

Topics

• Blotting, Southern
• Carcinoma, Squamous Cell (genetics)
• Chromosomes, Human, Pair 9 (genetics)
• DNA, Neoplasm (genetics)
• Esophageal Neoplasms (genetics)
• Gene Amplification
• Humans
• In Situ Hybridization, Fluorescence
• Jumonji Domain-Containing Histone Demethylases
• NFI Transcription Factors
• Neoplasm Proteins
• Proteins (genetics)
• Transcription Factors (genetics)
• Tumor Cells, Cultured