Neuropeptide receptor status in human tumor cell lines.

Abstract

Tumor types expressing a neuroendocrine phenotype secrete neuropeptides with paracrine or autocrine growth factor activity. The efficacy of these paracrine or autocrine loops depends on the expression of specific receptors on tumor cells. Once specific receptors are identified, specific neuropeptide antagonists disrupting paracrine and autocrine loops could be potential treatments in neuropeptide-secreting tumors. In the present study, 11 human tumor cell lines representing astrocytoma, lymphoma, and pancreatic, prostate, lung and colon carcinomas were examined for expression of five different neuropeptide receptors (cholecystokinin, neurotensin, vasopressin, tachykinine substance P and cannabinoid) using RT-PCR and radioligand binding. The presence of various neuropeptide receptors in different human cancer cell lines supports development of new antitumor treatments based on disruption of neuropeptide autocrine growth pathways.

• Authors: T Petit, K K Davidson, R A Lawrence, D D von Hoff, E Izbicka
• Journal: Anti-cancer drugs (Anticancer Drugs) Vol. 12 Issue 2 Pg. 133-6 (Feb 2001) ISSN: 0959-4973 [Print] England
• PMID: 11261886 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• RNA, Messenger
• RNA, Neoplasm
• Receptors, Neuropeptide

Topics

• Cell Division (drug effects)
• DNA Primers (chemistry)
• Humans
• RNA, Messenger (metabolism)
• RNA, Neoplasm (metabolism)
• Radioligand Assay
• Receptors, Neuropeptide (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured (metabolism)