Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability.

Abstract

In the present study, the effects of the combination of tamoxifen ((Z)-2[p-(1,2-diphenyl-1-butenyl)phenoxy]-N,N-dimethylamine citrate) and three cannabinoids (Delta(9)-tetrahydrocannabinol [Delta(9)-THC], cannabidiol, and anandamide [AEA]) upon the viability of C6 rat glioma cells was assessed at different incubation times and using different culturing concentrations of foetal bovine serum (FBS). Consistent with previous data for human glioblastoma cells, the tamoxifen sensitivity of the cells was increased as the FBS content of the culture medium was reduced from 10 to 0.4 and 0%. The cells expressed protein kinase C alpha and calmodulin (the concentration of which did not change significantly as the FBS concentration was reduced), but did not express estrogen receptors. Delta(9)-THC and cannabidiol, but not AEA, produced a modest reduction in cell viability after 6 days of incubation in serum-free medium, whereas no effects were seen in 10% FBS-containing medium. There was no observed synergy between the effects of tamoxifen and the cannabinoids upon cell viability.

• Authors: S O Jacobsson, E Rongård, M Stridh, G Tiger, C J Fowler
• Journal: Biochemical pharmacology (Biochem Pharmacol) Vol. 60 Issue 12 Pg. 1807-13 (Dec 15 2000) ISSN: 0006-2952 [Print] England
• PMID: 11108795 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Calmodulin
• Cannabinoids
• Isoenzymes
• Receptors, Estrogen
• Tamoxifen
• Protein Kinase C
• Protein Kinase C-alpha

Topics

• Animals
• Antineoplastic Agents, Phytogenic (pharmacology)
• Blood
• Calmodulin (biosynthesis)
• Cannabinoids (pharmacology)
• Cell Survival (drug effects)
• Drug Interactions
• Glioma (pathology)
• Isoenzymes (biosynthesis)
• Protein Kinase C (biosynthesis)
• Protein Kinase C-alpha
• Rats
• Receptors, Estrogen (biosynthesis)
• Tamoxifen (pharmacology)
• Tumor Cells, Cultured