Abstract |
Transforming growth factor ( TGF)-beta has been implicated in immunosuppression. However, it remains obscure whether regulation of T cells by TGF-beta contributes to the immunosuppression in vivo. To address this issue, we developed transgenic mice expressing Smad7, an intracellular antagonist of TGF-beta/Smad signaling, selectively in mature T cells using a plasmid construct coding a promoter element (the distal lck promoter) that directs high expression in peripheral T cells. Peripheral T cells were not growth inhibited by TGF-beta in Smad7 transgenic mice. Although Smad7 transgenic mice did not spontaneously show a specific phenotype, antigen-induced airway inflammation and airway reactivity were enhanced in Smad7 transgenic mice associated with high production of both T helper cell type 1 (Th1) and Th2 cytokines. Thus, blockade of TGF-beta/Smad signaling in mature T cells by expression of Smad7 enhanced airway inflammation and airway reactivity, suggesting that regulation of T cells by TGF-beta was crucial for negative regulation of the inflammatory (immune) response. Our findings also implicated TGF-beta/Smad signaling in mature T cells as a regulatory component of allergic asthma.
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Authors | A Nakao, S Miike, M Hatano, K Okumura, T Tokuhisa, C Ra, I Iwamoto |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 192
Issue 2
Pg. 151-8
(Jul 17 2000)
ISSN: 0022-1007 [Print] United States |
PMID | 10899902
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- DNA-Binding Proteins
- Smad7 Protein
- Smad7 protein, mouse
- Trans-Activators
- Transforming Growth Factor beta
- Ovalbumin
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Topics |
- Animals
- Asthma
(etiology)
- B-Lymphocytes
(physiology)
- Bronchial Hyperreactivity
(etiology)
- Cytokines
(biosynthesis)
- DNA-Binding Proteins
(physiology)
- Lymphocyte Activation
- Mice
- Mice, Transgenic
- Ovalbumin
(immunology)
- Smad7 Protein
- T-Lymphocytes
(physiology)
- Trachea
(pathology)
- Trans-Activators
(physiology)
- Transforming Growth Factor beta
(physiology)
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