Prognostic value of cell proliferation (Ki-67 antigen) and nuclear DNA content in clinically resectable, distal bile duct carcinoma.

Abstract

BACKGROUND:

The aim of this study was to investigate the prognostic value of cell proliferation (Ki-67 antigen) and DNA content in patients resected for distal bile duct carcinoma (DBDC).

METHODS:

Formalin-fixed tumor specimens of 35 patients with resected DBDC and a long-term clinical follow-up were analyzed. MIB-1 antibody was used for Ki-67 antigen detection to determine the proportion of proliferating cells. DNA content was measured using flow cytometry.

RESULTS:

A significant correlation was found between a low MIB-1 index (<20%) and survival (P <.05). Of the 35 tumor specimens, 34 specimens were evaluable by flow cytometry: 22 carcinomas were diploid (65%), and 12 were aneuploid (35%). The median DNA index of aneuploid tumors was 1.36 (range, 1.09 to 1.76). No correlation of DNA-ploidy with survival time was found.

CONCLUSION:

In contrast to DNA-ploidy pattern, Ki-67 antigen expression showed prognostic significance in resectable DBDC. A Ki-67 positive ratio of > or =20% was associated with decreased survival time.

• Authors: A M Rijken, A Umezawa, T M van Gulik, A Bosma, M M Polak, G J Offerhaus, H Obertop, D J Gouma
• Journal: Annals of surgical oncology (Ann Surg Oncol) Vol. 5 Issue 8 Pg. 699-705 (Dec 1998) ISSN: 1068-9265 [Print] United States
• PMID: 9869516 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Ki-67 Antigen

Topics

• Adult
• Aged
• Bile Duct Neoplasms (pathology, surgery)
• Cholangiocarcinoma (pathology, surgery)
• DNA, Neoplasm (analysis)
• Female
• Flow Cytometry
• Humans
• Immunohistochemistry
• Ki-67 Antigen (analysis)
• Male
• Middle Aged
• Prognosis
• Survival Analysis