Abstract |
We evaluated polymorphonuclear membrane (PMN) fluidity in 32 subjects with type 1 diabetes mellitus, 38 subjects with type 2 diabetes mellitus and 38 normal control subjects, by marking intact and unstimulated PMN cells with the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene ( TMA-DPH). We also evaluated PMN cytosolic Ca2+ content by marking intact and unstimulated PMN cells with the fluorescent probe Fura 2-AM. PMN membrane fluidity differentiated normal subjects from type 1 and 2 diabetic subjects. The PMN cytosolic Ca2+ concentration did not discriminate type 1 and 2 diabetic subjects from normal control subjects. No statistical correlation was found between PMN membrane fluidity and PMN cytosolic Ca2+ concentration in any of the groups of subjects, nor were significant correlations found between PMN membrane fluidity and cytosolic Ca2+ concentration in several plasma parameters (serum glucose, cholesterol and triglycerides). In conclusion, in type 1 and 2 diabetic patients we found a decrease in PMN membrane fluidity and this decrease, which was greater in type 2 diabetic patients, may be a marker of PMN dysfunction.
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Authors | G Caimi, B Canino, M Montana, G Ventimiglia, A Catania, R Lo Presti |
Journal | Acta diabetologica
(Acta Diabetol)
Vol. 35
Issue 3
Pg. 158-60
(Oct 1998)
ISSN: 0940-5429 [Print] Germany |
PMID | 9840453
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Fluorescent Dyes
- Glycated Hemoglobin A
- Triglycerides
- fura-2-am
- Cholesterol
- Calcium
- Fura-2
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Topics |
- Adult
- Blood Glucose
(metabolism)
- Calcium
(blood)
- Cholesterol
(blood)
- Cytosol
(metabolism)
- Diabetes Mellitus, Type 1
(blood)
- Diabetes Mellitus, Type 2
(blood)
- Female
- Fluorescent Dyes
- Fura-2
(analogs & derivatives)
- Glycated Hemoglobin
(analysis)
- Humans
- Male
- Membrane Fluidity
- Middle Aged
- Neutrophils
(physiology)
- Reference Values
- Triglycerides
(blood)
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