Regulation of infected-cell-specific protein synthesis in SF IPLB-21 cells productively infected with Spodoptera frugiperda multicapsid nuclear polyhedrosis virus.

Abstract

In Spodoptera frugiperda (SF IPLB-21) cells productively infected with S. frugiperda multicapsid nuclear polyhedrosis virus (SfMNPV), we observed the synthesis of infected-cell-specific polypeptides (ICSPs) using the protein synthesis inhibitor cycloheximide (CX) and the DAN synthesis inhibitor cytosine arabinoside (Ara-C) in an attempt to assess whether a temporal cascade and viral DNA synthesis are involved in the gene expression program of SfMNPV. Inhibition of protein synthesis with CX at 0 h postinfection resulted in the active synthesis of a group of ICSPs immediately after removal of CX, suggesting that these polypeptides, designated alpha-ICSP, did not require de novo protein synthesis for their production. A second group of ICSPs (designated beta-ICSPs), requiring a prior interval of protein synthesis, was detected when CX was added at later times. A third group of ICSPs also needed an interval of ICSP synthesis, but differed from beta-ICSPs in that they required a longer interval of protein synthesis. Moreover, the synthesis of most of these ICSPs also required DNA synthesis, which was demonstrated by addition of Ara-C before and after viral DNA replication; these ICSPs were therefore designated gamma-ICSPs. In conclusion, two kinds of regulatory processes are involved in SfMNPV infection: the first process controls the sequential synthesis of the tree ICSP groups, that is alpha-->beta-->gamma; a second process represses the synthesis of ICSP groups, first of the alpha-ICSPs and subsequently of beta-ICSPs. Thus, we propose temporally regulated as well as negative control circuits in SfMNPV gene expression.

• Authors: H S Liu, S L Bilimoria
• Journal: Intervirology (Intervirology) Vol. 40 Issue 1 Pg. 50-4 ( 1997) ISSN: 0300-5526 [Print] Switzerland
• PMID: 9268770 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Viral
• Nucleic Acid Synthesis Inhibitors
• Protein Synthesis Inhibitors
• Viral Proteins
• Cytarabine
• Cycloheximide

Topics

• Animals
• Cell Line
• Cycloheximide (pharmacology)
• Cytarabine (pharmacology)
• DNA, Viral (biosynthesis)
• Gene Expression Regulation, Viral (physiology)
• Nucleic Acid Synthesis Inhibitors (pharmacology)
• Nucleopolyhedroviruses (genetics, metabolism, physiology)
• Protein Synthesis Inhibitors (pharmacology)
• Spodoptera (virology)
• Viral Proteins (biosynthesis)
• Virus Replication (physiology)