Dietary calcium salts as protective agents and laminin P1 as a biochemical marker in chemically induced colon carcinogenesis in rats.

Abstract

To evaluate the protective effect of different calcium forms against colon carcinogenesis, Wistar rats fed a high-fat diet (24%) were supplemented with different chemical forms of dietary calcium and were intrarectally instilled with N-methyl-N-nitrosourea (NMU). Supplemental calcium was administered at 1.5% mineral (w/w of total diet) complexed with either carbonate, gluconate, or lactate in Groups 2, 3, and 4, respectively. The tumor incidence of colon cancer was compared with a control group (Group 1), fed the same diet without supplemental calcium. Colon carcinoma incidence was 31, 33, 13, and 7% in Groups 1, 2, 3, and 4, respectively. Calcium had a significant protective effect against carcinogenesis, and the maximum protective effect was observed with gluconate and lactate forms. Laminin P1 blood level was measured as a tumor marker. Laminin P1 results were compared with the reference group (Group T), fed a standard diet and not NMU instilled. The serum laminin P1 level was significantly higher (p = 0.0001) in NMU-instilled Groups 1, 2, 3, and 4 (0.24 +/- 0.03, 0.93 +/- 1.43, 0.84 +/- 1.33, and 0.41 +/- 0.34 mU/ml respectively) than in the Reference Group T (0.10 +/- 0.05 mU/ml).

• Authors: S Belbraouet, F Felden, X Pelletier, I Gastin, D Lambert, J Floquet, J L Guéant, G Debry
• Journal: Cancer detection and prevention (Cancer Detect Prev) Vol. 20 Issue 4 Pg. 294-9 ( 1996) ISSN: 0361-090X [Print] England
• PMID: 8818389 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Calcium, Dietary
• Laminin
• Peptide Fragments
• laminin P1

Topics

• Animals
• Biomarkers, Tumor (blood)
• Body Weight
• Calcium, Dietary (administration & dosage)
• Colonic Neoplasms (chemically induced, prevention & control)
• Laminin (blood)
• Male
• Peptide Fragments (blood)
• Rats
• Rats, Wistar